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2plb
From Proteopedia
(New page: 200px<br /><applet load="2plb" size="350" color="white" frame="true" align="right" spinBox="true" caption="2plb, resolution 1.60Å" /> '''D(GTATACC) under hyd...) |
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==Overview== | ==Overview== | ||
| - | The behaviour of the d(GGTATACC) oligonucleotide has been investigated by | + | The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage. |
==About this Structure== | ==About this Structure== | ||
| - | 2PLB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Spm Binding Site For Residue B 20'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLB OCA]. | + | 2PLB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SPM:'>SPM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:Spm+Binding+Site+For+Residue+B+20'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PLB OCA]. |
==Reference== | ==Reference== | ||
| - | Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007 Jul 7 | + | Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007;35(14):4800-8. Epub 2007 Jul 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17617642 17617642] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Fourme, R.]] | [[Category: Fourme, R.]] | ||
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[[Category: high-pressure]] | [[Category: high-pressure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:30:39 2008'' |
Revision as of 16:30, 21 February 2008
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D(GTATACC) under hydrostatic pressure of 1.39 GPa
Overview
The behaviour of the d(GGTATACC) oligonucleotide has been investigated by X-ray crystallography at 295 K in the range from ambient pressure to 2 GPa (approximately 20,000 atm). Four 3D-structures of the A-DNA form (at ambient pressure, 0.55, 1.09 and 1.39 GPa) were refined at 1.60 or 1.65 A resolution. In addition to the diffraction pattern of the A-form, the broad meridional streaks previously explained by occluded B-DNA octamers within the channels of the crystalline A-form matrix were observed up to at least 2 GPa. This work highlights an important property of nucleic acids, their capability to withstand very high pressures, while keeping in such conditions a nearly invariant geometry of base pairs that store and carry genetic information. The double-helix base-paired architecture behaves as a molecular spring, which makes it especially adapted to very harsh conditions. These features may have contributed to the emergence of a RNA World at prebiotic stage.
About this Structure
2PLB is a Protein complex structure of sequences from [1] with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Adaptation of the base-paired double-helix molecular architecture to extreme pressure., Girard E, Prange T, Dhaussy AC, Migianu-Griffoni E, Lecouvey M, Chervin JC, Mezouar M, Kahn R, Fourme R, Nucleic Acids Res. 2007;35(14):4800-8. Epub 2007 Jul 7. PMID:17617642
Page seeded by OCA on Thu Feb 21 18:30:39 2008
Categories: Protein complex | Fourme, R. | Girard, E. | Kahn, R. | Prange, T. | SPM | Crystal structure | Dna | High-pressure
