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1znm
From Proteopedia
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[[Category: zn-btd(7]] | [[Category: zn-btd(7]] | ||
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Revision as of 14:29, 30 October 2007
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A ZINC FINGER WITH AN ARTIFICIAL BETA-TURN, ORIGINAL SEQUENCE TAKEN FROM THE THIRD ZINC FINGER DOMAIN OF THE HUMAN TRANSCRIPTIONAL REPRESSOR PROTEIN YY1 (YING AND YANG 1, A DELTA TRANSCRIPTION FACTOR), NMR, 34 STRUCTURES
Overview
We have incorporated a bicyclic beta-turn mimetic (BTD; beta-turn, dipeptide) into a zinc finger, creating a zinc finger with an artificial, beta-turn. The designed peptide chelates zinc and has the same fold as the, unmodified native zinc finger (finger 3 of the human YY1 protein). A, combination of 1H NMR and structure calculations reveals that, in, solution, this zinc finger has a fold similar to the known wild-type, crystal structure and to other zinc fingers containing the consensus, sequence X3-Cys-X4-Cys-X12-His-X3-His-X. The peptide was designed with BTD, between the chelating cysteine residues, with BTD forming a type II', beta-turn linking the two strands of a distorted anti-parallel beta-sheet., The C-terminal portion of the peptide forms a helix with zinc, co-ordinating ... [(full description)]
About this Structure
1ZNM is a [Single protein] structure of sequence from [[1]] with ZN as [ligand]. Structure known Active Sites: BTD and ZNB. Full crystallographic information is available from [OCA].
Reference
Design, synthesis and structure of a zinc finger with an artificial beta-turn., Viles JH, Patel SU, Mitchell JB, Moody CM, Justice DE, Uppenbrink J, Doyle PM, Harris CJ, Sadler PJ, Thornton JM, J Mol Biol. 1998 Jun 19;279(4):973-86. PMID:9642075
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