2rfk
From Proteopedia
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==Overview== | ==Overview== | ||
| - | The most complex RNA pseudouridylases are H/ACA ribonucleoprotein | + | The most complex RNA pseudouridylases are H/ACA ribonucleoprotein particles, which use a guide RNA for substrate capture and four proteins (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we report the three-dimensional structure of a catalytically deficient archaeal enzyme complex (including the guide RNA and three of the four essential proteins) bound to a substrate RNA. Extensive interactions of Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked guide-substrate RNA complex structure and position the substrate in proximity of the Cbf5 catalytic center. Our structural and complementary fluorescence analyses also indicate that precise placement of the target uridine at the active site requires a conformation of the guide-substrate RNA duplex that is brought about by the previously identified concurrent interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Li, H.]] | [[Category: Li, H.]] | ||
[[Category: Liang, B.]] | [[Category: Liang, B.]] | ||
| - | [[Category: SECSG, Southeast | + | [[Category: SECSG, Southeast Collaboratory for Structural Genomics.]] |
| - | [[Category: Terns, M | + | [[Category: Terns, M P.]] |
| - | [[Category: Terns, R | + | [[Category: Terns, R M.]] |
[[Category: Xue, S.]] | [[Category: Xue, S.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: trna processing]] | [[Category: trna processing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:55 2008'' |
Revision as of 16:46, 21 February 2008
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Substrate RNA Positioning in the Archaeal H/ACA Ribonucleoprotein Complex
Overview
The most complex RNA pseudouridylases are H/ACA ribonucleoprotein particles, which use a guide RNA for substrate capture and four proteins (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we report the three-dimensional structure of a catalytically deficient archaeal enzyme complex (including the guide RNA and three of the four essential proteins) bound to a substrate RNA. Extensive interactions of Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked guide-substrate RNA complex structure and position the substrate in proximity of the Cbf5 catalytic center. Our structural and complementary fluorescence analyses also indicate that precise placement of the target uridine at the active site requires a conformation of the guide-substrate RNA duplex that is brought about by the previously identified concurrent interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process.
About this Structure
2RFK is a Protein complex structure of sequences from Pyrococcus furiosus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Substrate RNA positioning in the archaeal H/ACA ribonucleoprotein complex., Liang B, Xue S, Terns RM, Terns MP, Li H, Nat Struct Mol Biol. 2007 Dec 2;. PMID:18059286
Page seeded by OCA on Thu Feb 21 18:46:55 2008
Categories: Protein complex | Pyrococcus furiosus | Li, H. | Liang, B. | SECSG, Southeast Collaboratory for Structural Genomics. | Terns, M P. | Terns, R M. | Xue, S. | ZN | Archaeal h/aca ribonucleoprotein complex | Isomerase | Isomerase/rna complex | Protein-rna complex | Ribosome biogenesis | Rrna processing | Secsg | Southeast collaboratory for structural genomics | Structural genomics | Trna processing
