310d

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(New page: 200px<br /><applet load="310d" size="350" color="white" frame="true" align="right" spinBox="true" caption="310d, resolution 1.300&Aring;" /> '''CRYSTAL STRUCTURE O...)
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==Overview==
==Overview==
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The molecular and crystal structure of 2'-O-Me (CGCGCG)2 has been, determined using synchrotron radiation at near-atomic resolution (1.30 A), the highest resolution to date in the RNA field. The crystal structure is, a half-turn A-type helix with some helical parameters deviating from, canonical A-RNA, such as low base pair rise, elevated helical twist and, inclination angles. In CG steps, inter-strand guanines are parallel while, cytosines are not parallel. In steps GC this motif is reversed. This type, of regularity is not seen in other RNA crystal structures. The structure, includes 44 water molecules and two hydrated Mg2+ions one of which lies, exactly on the crystallographic 2-fold axis. There are distinct patterns, of hydration in the major and the minor grooves. The major groove is, stabilised by water clusters consisting of fused five- and six-membered, rings. Minor groove contains only a single row of water molecules; each, water bridges either two self-parallel cytosines or two self-parallel, guanines by a pair of hydrogen bonds. The structure provides the first, view of the hydration scheme of 2'-O-methylated RNA duplex.
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The molecular and crystal structure of 2'-O-Me (CGCGCG)2 has been determined using synchrotron radiation at near-atomic resolution (1.30 A), the highest resolution to date in the RNA field. The crystal structure is a half-turn A-type helix with some helical parameters deviating from canonical A-RNA, such as low base pair rise, elevated helical twist and inclination angles. In CG steps, inter-strand guanines are parallel while cytosines are not parallel. In steps GC this motif is reversed. This type of regularity is not seen in other RNA crystal structures. The structure includes 44 water molecules and two hydrated Mg2+ions one of which lies exactly on the crystallographic 2-fold axis. There are distinct patterns of hydration in the major and the minor grooves. The major groove is stabilised by water clusters consisting of fused five- and six-membered rings. Minor groove contains only a single row of water molecules; each water bridges either two self-parallel cytosines or two self-parallel guanines by a pair of hydrogen bonds. The structure provides the first view of the hydration scheme of 2'-O-methylated RNA duplex.
==About this Structure==
==About this Structure==
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Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA., Adamiak DA, Milecki J, Popenda M, Adamiak RW, Dauter Z, Rypniewski WR, Nucleic Acids Res. 1997 Nov 15;25(22):4599-607. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9358171 9358171]
Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA., Adamiak DA, Milecki J, Popenda M, Adamiak RW, Dauter Z, Rypniewski WR, Nucleic Acids Res. 1997 Nov 15;25(22):4599-607. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9358171 9358171]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Adamiak, D.A.]]
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[[Category: Adamiak, D A.]]
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[[Category: Adamiak, R.W.]]
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[[Category: Adamiak, R W.]]
[[Category: Dauter, Z.]]
[[Category: Dauter, Z.]]
[[Category: Milecki, J.]]
[[Category: Milecki, J.]]
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[[Category: Rypniewski, W.R.]]
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[[Category: Rypniewski, W R.]]
[[Category: MG]]
[[Category: MG]]
[[Category: a-rna]]
[[Category: a-rna]]
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[[Category: modified]]
[[Category: modified]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:34:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:01:52 2008''

Revision as of 17:01, 21 February 2008

Image:310d.jpg

310d, resolution 1.300Å

Drag the structure with the mouse to rotate

CRYSTAL STRUCTURE OF 2'-O-ME(CGCGCG)2: AN RNA DUPLEX AT 1.3 A RESOLUTION. HYDRATION PATTERN OF 2'-O-METHYLATED RNA

Overview

The molecular and crystal structure of 2'-O-Me (CGCGCG)2 has been determined using synchrotron radiation at near-atomic resolution (1.30 A), the highest resolution to date in the RNA field. The crystal structure is a half-turn A-type helix with some helical parameters deviating from canonical A-RNA, such as low base pair rise, elevated helical twist and inclination angles. In CG steps, inter-strand guanines are parallel while cytosines are not parallel. In steps GC this motif is reversed. This type of regularity is not seen in other RNA crystal structures. The structure includes 44 water molecules and two hydrated Mg2+ions one of which lies exactly on the crystallographic 2-fold axis. There are distinct patterns of hydration in the major and the minor grooves. The major groove is stabilised by water clusters consisting of fused five- and six-membered rings. Minor groove contains only a single row of water molecules; each water bridges either two self-parallel cytosines or two self-parallel guanines by a pair of hydrogen bonds. The structure provides the first view of the hydration scheme of 2'-O-methylated RNA duplex.

About this Structure

310D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of 2'-O-Me(CGCGCG)2, an RNA duplex at 1.30 A resolution. Hydration pattern of 2'-O-methylated RNA., Adamiak DA, Milecki J, Popenda M, Adamiak RW, Dauter Z, Rypniewski WR, Nucleic Acids Res. 1997 Nov 15;25(22):4599-607. PMID:9358171

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