351d
From Proteopedia
(New page: 200px<br /><applet load="351d" size="350" color="white" frame="true" align="right" spinBox="true" caption="351d, resolution 1.640Å" /> '''X-RAY CRYSTAL STRUC...) |
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==Overview== | ==Overview== | ||
| - | Hexammine ions are strong inducers of the transition from the B-form to | + | Hexammine ions are strong inducers of the transition from the B-form to the left-handed Z-form in DNA. Here the structure of d(CACGCG). d(CGCGTG) obtained from crystals grown from a drop containing [Ru(NH3)6]Cl3 is reported. The structure is clearly characterized as Z-DNA. When compared with the structure of d(CACGCG).d(CGCGTG)/MgCl2 and that of d(CGCGCG)2, subtle differences are seen, most noticeably in the water structure. Since stable well diffracting crystals grow easily in the presence of [Ru(NH3)6]Cl3 and since this ion is not visible in the electron density it is concluded that the ion plays a non-specific role in stabilizing Z-DNA. |
==About this Structure== | ==About this Structure== | ||
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[[Category: z-dna]] | [[Category: z-dna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:15 2008'' |
Revision as of 17:02, 21 February 2008
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X-RAY CRYSTAL STRUCTURES OF THE HEXAMER DCACGCG: CRYSTALS GROWN IN THE PRESENCE OF RUTHENIUM (II) HEXAMMINE CHLORIDE
Overview
Hexammine ions are strong inducers of the transition from the B-form to the left-handed Z-form in DNA. Here the structure of d(CACGCG). d(CGCGTG) obtained from crystals grown from a drop containing [Ru(NH3)6]Cl3 is reported. The structure is clearly characterized as Z-DNA. When compared with the structure of d(CACGCG).d(CGCGTG)/MgCl2 and that of d(CGCGCG)2, subtle differences are seen, most noticeably in the water structure. Since stable well diffracting crystals grow easily in the presence of [Ru(NH3)6]Cl3 and since this ion is not visible in the electron density it is concluded that the ion plays a non-specific role in stabilizing Z-DNA.
About this Structure
351D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of d(CACGCG).d(CGCGTG) in crystals grown in the presence of ruthenium III hexammine chloride., Karthe P, Gautham N, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):501-9. PMID:9761846
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