This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
398d
From Proteopedia
(New page: 200px<br /> <applet load="398d" size="450" color="white" frame="true" align="right" spinBox="true" caption="398d, resolution 1.940Å" /> '''3'-DNA-RNA-5' JUNC...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:398d.gif|left|200px]]<br /> | + | [[Image:398d.gif|left|200px]]<br /><applet load="398d" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="398d" size=" | + | |
caption="398d, resolution 1.940Å" /> | caption="398d, resolution 1.940Å" /> | ||
'''3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION'''<br /> | '''3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | During initiation of minus-strand synthesis by HIV-1 reverse | + | During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H. |
==About this Structure== | ==About this Structure== | ||
| - | 398D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 398D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=398D OCA]. |
==Reference== | ==Reference== | ||
| Line 21: | Line 20: | ||
[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:37 2008'' |
Revision as of 17:02, 21 February 2008
|
3'-DNA-RNA-5' JUNCTION FORMED DURING INITIATION OF MINUS-STRAND SYNTHESIS OF HIV REPLICATION
Overview
During initiation of minus-strand synthesis by HIV-1 reverse transcriptase, a 3'-DNA-RNA-5' junction is formed involving the 3'-end of tRNAlys,3. The HIV-RT-associated RNase H cleaves the RNA template strand specifically, opposite the newly synthesized DNA strand. We have determined the crystal structure at 1.9 A resolution of an eight-base pair hybrid duplex representing the junction to identify global or local structural perturbations which may be recognized by HIV-RT RNase H. The junction octamer is in a global A-type conformation throughout. A base pair step with distinct stacking geometry and variable backbone conformation is located next to the main endonucleolytic cleavage site. This base pair step may serve as a recognition site for HIV-RT RNase H.
About this Structure
398D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of an eight-base pair duplex containing the 3'-DNA-RNA-5' junction formed during initiation of minus-strand synthesis of HIV replication., Mueller U, Maier G, Mochi Onori A, Cellai L, Heumann H, Heinemann U, Biochemistry. 1998 Sep 1;37(35):12005-11. PMID:9724510
Page seeded by OCA on Thu Feb 21 19:02:37 2008
