Beta-lactamase

From Proteopedia

Revision as of 11:25, 12 February 2014

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Beta-lactamase (Blac) are enzymes which make bacteria resistant to β-lactam antibiotics by breaking their β-lactam ring. Blac are classified according to their resistance targets. Class C are cephalosporinases; Class A are broad-spectrum; Class B are metalloenzymes; Class D are cloxacilanases. Carbapenemase (Car) are Blac with wide hydrolytic capacities. Car can hydrolyze penicillins, cephalosporins, monobactams and carbapenems. Extended-spectrum Blac class A (ESBL) hydrolyze penicillins but not cephalosporins.

3D Structures of Beta-lactamase

Updated on 12-February-2014

β-lactamase class A

3dtm, 1bza, 1ck3, 1esu, 1jwp, 1lhy, 1li0, 1m40, 1zg6, 2v1z, 2v20, 3cmz, 3jyi, 4ibr, 4ibx - EcBlac A Tem-1 (mutant) - Escherichia coli
1btl, 1iys, 1xpb, 1zg4, 4gku - EcBlac A Tem-1
1lhy - EcBlac A Tem-30 (mutant)
1li0 - EcBlac A Tem-32 (mutant)
1li9 - EcBlac A Tem-34 (mutant)
1yt4 - EcBlac A Tem-76 (mutant)
3toi - EcBlac A Tem-116 (mutant)
4id4 – EcBlac/PaBlac Tem
1we4, 2xr0, 2zq7, 2zq8, 2zq9, 2zqa, 2zqc, 2zqd, 4bd0, 4bd1 - EcBlac A Toho-1 (mutant)
2xqz, 2wyx - EcBlac A Toho-1 (mutant) – Neutron
3c5a – EcBlac A Kpc-2
1ylj, 1ylp, 1ylt, 1ylw, 1yly, 1ylz, 1ym1, 1yms, 1ymx, 2p74 – EcBlac A Ctx-M
3hre - EcBlac A Ctx-M (mutant)
3o3v – EcBlac peptidase domain
1o7e, 1n4o – StmBlac A – Stenotrophonas maltophilia
1htz – KpBlac A Tem-52 – Klebsiella pneumoniae
1shv, 4fh4, 4fcf, 4jpm - KpBlac A Shv-1
1tdl, 2a3u, 3oph, 3opl, 3v50, 3v5m, 4fd8 - KpBlac A Shv-1 (mutant)
1n9b – KpBlac A Shv-2
2qpn – KpBlac A Ges-1
3ni9 - PaBlac A Ges-2
1hzo – Blac A – Proteus vulgaris
1kge, 1kgf, 1kgg, 1ome– SaBlac A (mutant) - Staphylococcus aureus
3blm – SaBlac A
1mbl, 3ly3, 3soi – BlBlac A (mutant) – Bacillus licheniformis
2blm, 3sh7, 4blm – BlBlac A
2cc1 – Blac A – Mycobacterium fortuitum
3bfd, 3bfe – CsBlac A Sed-1 (mutant) - Citrobacter sedlakii
3s4x - EncBlac A (mutant) – Enterobacter cloacae
3zdj, 4b88 – Blac – precambrian

Extended-spectrum β-lactamase class A

4gog - PaESBL A GES-1 + acyl intermediate
3nia - PaESBL A GES-2 + inhibitor
4gnu, 4h8r - PaESBL A GES-5
3v3s – PaESBL GES-18
1e25 – PaESBL PER-1 (mutant)
3znw - CfESBL PER-2 + antribiotic – Citrobacter freundii
3v3r - AbESBL A GES-11– Acinetobacter baumannii
3tsg – AbESBL GES-14
2qpn – KpESBL A GES-1
3ni9 - PaESBL A GES-2
3g2y, 3g2z, 3g30, 3g31, 3g32, 3g34, 3g35, 4dds, 4ddy, 4de0, 4de1, 4de2, 4de3 - EcBlac A Ctx-M-9 + inhibitor
3hlw, 3huo, 3hvf - EcBlac A Ctx-M-9 (mutant) + antibiotic
4hbt – EcESBL Ctx-M-15
4hbu – EcESBL Ctx-M-15 + inhibitor

β-lactamase class A binary complex

1axb - EcBlac A (mutant) + transition state analog
1tem, 1bt5, 1fqg, 1iyo, 1iyp, 1iyq, 1jvj, 3q07, 3q1f - EcBlac A (mutant) + antibiotic
1nxy, 1ny0, 1nym, 1nyy - EcBlac A (mutant) + boronic acid inhibitor
1pzo, 1pzp - EcBlac A Tem-1 (mutant) + inhibitor
1erm, 1ero, 1erq – EcBlac A Tem-1 + boronic acid inhibitor
1jwv, 1jwz - EcBlac A Tem-1 (mutant) + boronic acid inhibitor
1ong, 1q2p, 1vm1, 2h5s, 2zd8, 3d4f, 3mke, 3mkf, 3mxr, 3mxs, 4fh2, 4gd6, 4gd8, 4gdb - KpBlac A Shv-1 + inhibitor
1rcj, 1tdg, 2a49, 2h0t, 2h0y, 2h10 - KpBlac A Shv-1 (mutant) + inhibitor
3opp, 3opr- KpBlac A Shv-1 (mutant) + antibiotic
1w7f, 3b3x, 2y91, 4a5r - BlBlac A + inhibitor
3m2j - BlBlac A (mutant) + inhibitor
2wk0, 2x71– BlBlac A + antibiotic
3ly4, 3m2k, 3sh8, 3sh9 - BlBlac A (mutant) + antibiotic
2q9m, 2q9n - EncBlac A + inhibitor
3bfc, 3bff, 3bfg – CsBlac A Sed-1 (mutant) + inhibitor
3nia - PaBlac A Ges-2 + inhibitor
3p98 – Blac Tem-72 + citrate – Morganella morganii

β-lactamase class A complex with β-lactamase inhibitor protein (Blip)

3c7u, 3c7v, 1jtg, 2b5r – EcBlac A (mutant) + Protein Blip
1jtd, 3gmw - EcBlac A + Protein Blip
1s0w, 1xxm - EcBlac A + Protein Blip (mutant)
2g2u - KpBlac A Shv-1 + Protein Blip
2g2w, 3n4i - KpBlac A Shv-1 (mutant) + Protein Blip
3c4o, 3c4p - KpBlac A Shv-1 + Protein Blip (mutant)
3e2k, 3e2l - KpBlac A Kpc-2 (mutant) + Protein Blip
3qhv, 3qhy - Blac A + Protein Blip – Bacillus anthracis

β-lactamase class B (Zn containing)

3fcz, 3i0v, 3i11, 3i13, 3i14, 3i15, 1bc2, 1bvt, 1mqo, 2bc2, 3bc2, 1bmc – BcBlac B - Bacillus cereus
2m5c - BcBlac B – NMR
3knr, 3kns, 1dxk, 2bfk, 2bfl, 2bfz, 2bg2, 2bg6, 2bg7, 2bg8, 2bga, 2nxa, 2nyp, 2nze, 2nzf, 2uyx - BcBlac B (mutant)
1a7t, 1znb, 2bmi – BfBlac B – Bacterioides fragilis
2znb - BfBlac B (Cd)
3znb – BfBlac B (Zn, Hg)
4znb - BfBlac B (mutant)
1k07 – FgBlac B]] - Fluoribacter gormanii
1l9y - FgBlac B (mutant)
1ko2, 1ko3 – PaBlac B Vim-2 - Pseudomonas aeruginosa
2whg, 2wrs - PaBlac B Vim-4
4awy, 4awz - PaBlac B Aim-1
4ax0, 4ax1 - PaBlac B Aim-1 (mutant)
2y87, 2y8a, 2y8b - PaBlac B Vim-7
2fhx - PaBlac B Spm-1
2x01 - PaBlac Oxa-10 (mutant)
2x02 - PaBlac Oxa-10
4f6h, 4f6z – PaBlac Imp-1 (mutant)
1wuo, 1wup - SmBlac Imp-1 (mutant) – Serratia marcescens
3vpe - SmBlac Smb-1
2dkf, 2zo4, 2zwr – TtBlac B – Thermus thermophilus
2zzi – TtBlac B (Fe)
2fm6, 2fu6, 2fu7, 2h6a – StmMlac B
2qin, 2qjs - StmBlac B (mutant)
2gmn, 3lvz – BjBlac B – Bradyrhizobium japonicum
2p4z – Blac B – Thermoanaerobacter tengcongensis
3l6n – Blac B – Chryseobacter indolegenes
3r2u – SaBlac B
3rkj, 3sbl, 3sfp, 3spu, 3zr9 - KpBlac B Ndm-1
3srx - KpBlac B Ndm-1 (Cd)
4h0d - KpBlac B Ndm-1 (Mn)
3rkk, 4gyq, 4gyu - KpBlac B Ndm-1 (mutant)
3s0z - EcBlac B Ndm-1
2yz3 – Blac Vim-2 – Pseudomonas putida
3sd9 – SfBlac B - Serratia fonticola
3pg4 – KpBlac Ndm-1

β-lactamase class B binary complex

1a8t, 1hlk, 1kr3 - BfBlac B + inhibitor
1jt1 – FgBlac + captopril
1jje, 1jjt, 3lce - PaBlac B + inhibitor
1m2x – Blac B + captopril – Elizabethkingia meningoseptica
1vgn, 3vqz - SmBlac Imp-1+ inhibitor
2doo - SmBlac Imp-1+ fluorescent agent
2aio – StmBlac B + antibiotic
2fu8, 2fu9, 2gfj, 2gfk, 2hb9, 2qdt - StmBlac B + inhibitor
3iof, 1x8i, 3iog– AhBlac B (mutant) + inhibitor
1x8g, 2gkl – AhBlac B + inhibitor
2qds – AhBlac B + captopril
3m8t – BjBlac B + inhibitor
2m5d - BcBlac B + inhibitor
3q6v – SfBlac B + glycerol
3q6x, 4ey2, 4eyb, 4eyf, 4efl, 4hl1, 4hl2 – KpBlac B Ndm-1+ antibiotic
4exy, 4exs, 4eyl, 4hky – KpBlac B Ndm-1+ inhibitor

β-lactamase class C

3fkw, 3iwi, 3iwo, 3iwq, 3ixd – EcBlac C (mutant)
1zkj – Blac C – Enterobacter aerogenes
2qz6 – Blac C – Pseudomonas fluorescens

β-lactamase class C binary complex

3bm6, 3fkv, 3o86, 3o87, 3o88 – EcBlac C + boronic acid inhibitor
3ixb, 3ixg - EcBlac C (mutant) + boronic acid inhibitor
3ixh - EcBlac C (mutant) + cefotaxime
3gqz, 3gr2, 3grj, 3gsg, 3gtc, 3gv9, 3gvb - EcBlac C + inhibitor
1onh, 1q2q, 1y54 - EncBlac C + inhibitor
1s6r - EncBlac C+ boronic acid inhibitor
1rgz - EncBlac C + antribiotic
1rgy - CfBlac C + antibiotic
1zc2, 2zc7 – KpBlac C + inhibitor
2wzx, 2wzz – PaBlac C + inhibitor

β-lactamase class D

2rl3, 1k55, 1k57, 1k4e, 1ewz, 1fof, 1ddk, 1e3u, 1e4d, 1h8z – PaBlac D
2hp5, 2hp6, 2hp9, 2hpb, 2wgw, 2wki - PaBlac D (mutant)
1k56, 2k02 - PaBlac Pse-2
1g68, 1k4f – PaBlac Pse-4
1g6a – PaBlac Pse-4 (mutant)
1ko2 – PaBlac Vim-2
1k38 – Blac D – Salmonella typhimurium
1m6k – EcBlac D
4mll - KpBlac D Oxa-1 + oxacillin
3hbr – KpBlac D Oxa-48
3if6 - KpBlac D Oxa-46
4ied – Blac – Fusobacterium nucleatum

β-lactamase class D binary complex

1k6r, 1k54 - PaBlac D + antibiotic
2wgi, 2wkh - PaBlac D (mutant) + antibiotic
1k6s - PaBlac D + phenylboronic acid
1dd6, 1h5x, 1h8y, 2wgv - PaBlac D + inhibitor
3isg – EcBlac D + inhibitor
3pae, 3pag, 4f94 - AbBlac D (mutant) + inhibitor
3znt - AbBlac D + inhibitor

AmpC β-lactamase

1ke4, 2bls, 2zj9, 3bls – EcBlac
1l0d, 1l0e, 1l0f, 1l0g - EcBlac (mutant) 1fcn – EcBlac (mutant) + substrate
1fco, 1i5q, 1kvl - EcBlac (mutant) + antibiotic
1iel, 1fcm, 1iem, 1kvm, 1ll5, 1ll9, 1llb - EcBlac + antibiotic
1fsw, 1fsy, 1kds, 1kdw, 1ke0, 1ke3, 1mxo, 1my8, 4e3i, 4e3j, 4e3k, 4e3l, 4e3m, 4e3n, 4e3o, 2i72 - EcBlac + boronic acid inhibitor
1pi4, 1pi5, 2ffy - EcBlac (mutant) + boronic acid inhibitor
1l2s, 1xgi, 1xgj, 2hdq, 2hdr, 2hds, 2hdu, 2p9v, 2pu2, 2pu4, 2r9w, 2r9x, 2rcx - EcBlac + inhibitor
1o07 - EcBlac (mutant) + inhibitor
3s1y, 3s22 - PaBlac (mutant) + inhibitor
4hef - PaBlac + inhibitor
4gzb – PaBlac

Carbapenemase

3f9o - AhBlac B – Aeromonas hydrophila
3fai, 1x8h, 3sw3, 3t9m - AhBlac B (mutant)
3rxw, 3rxx – KpBlac Kpc
2ov5 - KpBlac A Kpc
4eqi – SfBlac Sfc-1
4euz, 4ev4 – SfBlac Sfc-1 (mutant) + antibiotic
4jf5, 4jf6 - AbBlac OXA-23
4jf4 - AbBlac OXA-23 + antibiotic
1bue – EncBlac

Non-classified β-lactamase

1bsg – Blac – Streptomyces albus
1alq, 1blp, 1dja, 1djb, 1djc, 1ghi – SaBlac (mutant)
1gce, 1xx2 - EncBlac
4fr7, 4fsb - EncBlac Vim-31 (mutant)
1dy6 – SmBlac Sme-1
1fr1, 1fr6 – CfBlac
1i2s – BlBlac
2gdn, 3zhh – MtBlac – Mycobacterium tuberculosis
4jlf - MtBlac (mutant)
3vff, 3vfh, 4ebl, 4ebn, 4ebp – MtBlac (mutant) + intermediate
4df6 – MtBlac + inhibitor
2jc7, 3g4p – AbBlac
3byd – Blac – Klebsiella oxytoca
3i7j – Blac – Mycobacterium bovis
3lez – Blac – Oceanobacter iheyensis
3p09 – Blac – Francisella tularensis
4blm - BlBlac
4ewf – Blac – Sphaerobacter thermophilus
3w4o, 3w4p, 4efz – Blac – Burkholderia pseudomallei
3w4q – Blac – Burkholderia multivorans
4k0w – AbBlac Oxa-23 (mutant)
4k0x – AbBlac Oxa-23

Non-classified β-lactamase binary complex

1ghm, 1ghp, 1pio - SaBlac (mutant) + antibiotic
1blc, 1blh– SaBlac + inhibitor
1bls – EncBlac + phosphonate derivative
1bul - EncBlac + penicillin derivative
1ga0 - EncBlac + inhibitor
1i2w – BlBlac + cifoxitin
3cg5 – MtBlac + antibiotic
3n8s, 3ny4, 3n6i, 3n7w, 3n8l, 3n8r, 3nbl, 3nc8, 3nck, 3nde, 3ndg - MtBlac (mutant) + antibiotic
3dwz, 3iqa, 3m6b, 3m6h – MtBlac + inhibitor
3fv7, 3fyz, 3fzc, 3mbz – AbBlac + inhibitor