3fct
From Proteopedia
(New page: 200px<br /> <applet load="3fct" size="450" color="white" frame="true" align="right" spinBox="true" caption="3fct, resolution 2.40Å" /> '''MATURE METAL CHELAT...) |
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| - | [[Image:3fct.gif|left|200px]]<br /> | + | [[Image:3fct.gif|left|200px]]<br /><applet load="3fct" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="3fct" size=" | + | |
caption="3fct, resolution 2.40Å" /> | caption="3fct, resolution 2.40Å" /> | ||
'''MATURE METAL CHELATASE CATALYTIC ANTIBODY WITH HAPTEN'''<br /> | '''MATURE METAL CHELATASE CATALYTIC ANTIBODY WITH HAPTEN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A classic hypothesis for enzyme catalysis is the induction of strain in | + | A classic hypothesis for enzyme catalysis is the induction of strain in the substrate. This notion was first expressed by Haldane with the lock and key analogy-"the key does not fit the lock perfectly but exercises a certain strain on it" (1). This mechanism has often been invoked to explain the catalytic efficiency of enzymes but has been difficult to establish conclusively (2-7). Here we describe X-ray crystallographic and mutational studies of an antibody metal chelatase which strongly support the notion that this antibody catalyzes metal ion insertion into the porphyrin ring by inducing strain. Analysis of the germline precursor suggests that this strain mechanism arose during the process of affinity maturation in response to a conformationally distorted N-alkylmesoporphyrin. |
==About this Structure== | ==About this Structure== | ||
| - | 3FCT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CD, CA, MG, NA and MMP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 3FCT is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CD:'>CD</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NA:'>NA</scene> and <scene name='pdbligand=MMP:'>MMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FCT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Barnes, D.]] | [[Category: Barnes, D.]] | ||
| - | [[Category: Romesberg, F | + | [[Category: Romesberg, F E.]] |
| - | [[Category: Santarsiero, B | + | [[Category: Santarsiero, B D.]] |
| - | [[Category: Schultz, P | + | [[Category: Schultz, P G.]] |
[[Category: Spiller, B.]] | [[Category: Spiller, B.]] | ||
| - | [[Category: Stevens, R | + | [[Category: Stevens, R C.]] |
[[Category: Yin, J.]] | [[Category: Yin, J.]] | ||
[[Category: CA]] | [[Category: CA]] | ||
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[[Category: metal chelatase]] | [[Category: metal chelatase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:09:21 2008'' |
Revision as of 17:09, 21 February 2008
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MATURE METAL CHELATASE CATALYTIC ANTIBODY WITH HAPTEN
Overview
A classic hypothesis for enzyme catalysis is the induction of strain in the substrate. This notion was first expressed by Haldane with the lock and key analogy-"the key does not fit the lock perfectly but exercises a certain strain on it" (1). This mechanism has often been invoked to explain the catalytic efficiency of enzymes but has been difficult to establish conclusively (2-7). Here we describe X-ray crystallographic and mutational studies of an antibody metal chelatase which strongly support the notion that this antibody catalyzes metal ion insertion into the porphyrin ring by inducing strain. Analysis of the germline precursor suggests that this strain mechanism arose during the process of affinity maturation in response to a conformationally distorted N-alkylmesoporphyrin.
About this Structure
3FCT is a Protein complex structure of sequences from Homo sapiens with , , , and as ligands. Full crystallographic information is available from OCA.
Reference
Structural and kinetic evidence for strain in biological catalysis., Romesberg FE, Santarsiero BD, Spiller B, Yin J, Barnes D, Schultz PG, Stevens RC, Biochemistry. 1998 Oct 13;37(41):14404-9. PMID:9772166
Page seeded by OCA on Thu Feb 21 19:09:21 2008
Categories: Homo sapiens | Protein complex | Barnes, D. | Romesberg, F E. | Santarsiero, B D. | Schultz, P G. | Spiller, B. | Stevens, R C. | Yin, J. | CA | CD | MG | MMP | NA | Catalytic antibody | Fab fragment | Metal chelatase
