Horseradish peroxidase
From Proteopedia
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[[1atj]], [[1h5d]], [[1h5e]], [[1h5f]], [[1h5g]], [[1g5i]], [[1g5j]], [[1g5k]], [[1g5m]], [[1g57]], [[1h55]], [[1h58]], [[1h5c]], [[1h5h]], [[1h5l]], [[1hch]] – HRP C1a<br /> | [[1atj]], [[1h5d]], [[1h5e]], [[1h5f]], [[1h5g]], [[1g5i]], [[1g5j]], [[1g5k]], [[1g5m]], [[1g57]], [[1h55]], [[1h58]], [[1h5c]], [[1h5h]], [[1h5l]], [[1hch]] – HRP C1a<br /> | ||
| - | [[1kzm]], [[1gwo]], [[1gwt]], [[1gwu]] - HRP C1a (mutant) | + | [[1kzm]], [[1gwo]], [[1gwt]], [[1gwu]], [[2ylj]] - HRP C1a (mutant) |
'''Horseradish peroxidase binary complex''' | '''Horseradish peroxidase binary complex''' | ||
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[[1h5a]] – HRP C1a + acetate<br /> | [[1h5a]] – HRP C1a + acetate<br /> | ||
[[1w4w]] - HRP C1a + formate<br /> | [[1w4w]] - HRP C1a + formate<br /> | ||
| - | [[ | + | [[1w4y]] - HRP C1a + CO |
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 08:44, 18 March 2014
Horseradish peroxidase (HRP) catalyzes the oxidation of luminol to 3-aminophthalate using hydrogen peroxide. The reaction is accompanied by emission of light. In the presence of chemicals, particularly – p-iodophenol, the light emission is enhanced 1000-fold. HRP isozyme C1a is widely used for detection of a labeled molecule to which it conjugates. Upon addition of the proper substrate, the HRP complex emits colored light. HRP contains a heme group.
3D structures of horseradish peroxidase
Updated on 18-March-2014
1atj, 1h5d, 1h5e, 1h5f, 1h5g, 1g5i, 1g5j, 1g5k, 1g5m, 1g57, 1h55, 1h58, 1h5c, 1h5h, 1h5l, 1hch – HRP C1a
1kzm, 1gwo, 1gwt, 1gwu, 2ylj - HRP C1a (mutant)
Horseradish peroxidase binary complex
2atj – HRP C1a + benzhydroxamic acid
3atj, 4atj, 1gx2 - HRP C1a (mutant) + benzhydroxamic acid
6atj - HRP C1a + ferulic acid
1gw2 - HRP C1a (mutant) + ferulic acid
7atj - HRP C1a + CN + ferulic acid
1h5a – HRP C1a + acetate
1w4w - HRP C1a + formate
1w4y - HRP C1a + CO
