3ung
From Proteopedia
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{{STRUCTURE_3ung| PDB=3ung | SCENE= }} | {{STRUCTURE_3ung| PDB=3ung | SCENE= }} | ||
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===Structure of the Cmr2 subunit of the CRISPR RNA silencing complex=== | ===Structure of the Cmr2 subunit of the CRISPR RNA silencing complex=== | ||
| + | {{ABSTRACT_PUBMED_22405013}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/CMR2_PYRFU CMR2_PYRFU]] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage. Probably not the subunit that cleaves pre-crRNA, as mutation of numerous metal-binding residues have no effect on cleavage by assembled complex.<ref>PMID:19945378</ref> | |
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==About this Structure== | ==About this Structure== | ||
| - | [[3ung]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | + | [[3ung]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrfu Pyrfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UNG OCA]. |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID:022405013</ref><references group="xtra"/> | + | <ref group="xtra">PMID:022405013</ref><references group="xtra"/><references/> |
| - | [[Category: | + | [[Category: Pyrfu]] |
[[Category: Cocozaki, A I.]] | [[Category: Cocozaki, A I.]] | ||
[[Category: Hale, C R.]] | [[Category: Hale, C R.]] | ||
Revision as of 07:55, 2 April 2014
Contents |
Structure of the Cmr2 subunit of the CRISPR RNA silencing complex
Template:ABSTRACT PUBMED 22405013
Function
[CMR2_PYRFU] CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA), formerly called psiRNA (prokaryotic silencing) in this organism. Part of the Cmr ribonucleoprotein complex which has divalent cation-dependent endoribonuclease activity specific for ssRNA complementary to the crRNA, generating 5' hydroxy- and 3' phosphate or 2'-3' cyclic phosphate termini. It is not known which subunit has endoribonuclease activity. Cmr complex does not cleave ssDNA complementary to the crRNA. Cleavage of invading RNA is guided by the crRNA; substrate cleavage occurs a fixed distance (14 nt) from the 3' end of the crRNA. In vitro reconstitution shows Cmr1-2 and Cmr5 are not necessary for cleavage. Probably not the subunit that cleaves pre-crRNA, as mutation of numerous metal-binding residues have no effect on cleavage by assembled complex.[1]
About this Structure
3ung is a 1 chain structure with sequence from Pyrfu. Full crystallographic information is available from OCA.
Reference
- Cocozaki AI, Ramia NF, Shao Y, Hale CR, Terns RM, Terns MP, Li H. Structure of the Cmr2 Subunit of the CRISPR-Cas RNA Silencing Complex. Structure. 2012 Mar 7;20(3):545-53. PMID:22405013 doi:10.1016/j.str.2012.01.018
- ↑ Hale CR, Zhao P, Olson S, Duff MO, Graveley BR, Wells L, Terns RM, Terns MP. RNA-guided RNA cleavage by a CRISPR RNA-Cas protein complex. Cell. 2009 Nov 25;139(5):945-56. doi: 10.1016/j.cell.2009.07.040. PMID:19945378 doi:10.1016/j.cell.2009.07.040
