2k0b
From Proteopedia
(New page: 200px<br /><applet load="2k0b" size="350" color="white" frame="true" align="right" spinBox="true" caption="2k0b" /> '''NMR structure of the UBA domain of p62 (SQST...) |
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'''NMR structure of the UBA domain of p62 (SQSTM1)'''<br /> | '''NMR structure of the UBA domain of p62 (SQSTM1)'''<br /> | ||
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| + | ==Overview== | ||
| + | The ubiquitin associated domain of p62 is a small three-helix bundle of approximately 50 residues that mediates the recognition of polyubiquitin chains and ubiquitylated substrates. The solution structure of a 52 residue construct containing this domain has been characterized using heteronuclear nuclear magnetic resonance (NMR) methods. The resulting ensemble of NMR-derived structures was used in molecular dynamics (MD) simulations to investigate the equilibrium conformation and dynamics of this domain. NOE and (15)N relaxation data have been used to validate the structural ensemble produced by the MD simulations and show a good correlation for residues in regions of secondary structure. A similar approach was taken using an ensemble of structures from the MD simulations to calculate electronic circular dichroism (CD) and IR spectra from first principles with an encouraging correlation with the experimental CD and IR data. Proteins 2008. (c) 2007 Wiley-Liss, Inc. | ||
==About this Structure== | ==About this Structure== | ||
2K0B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0B OCA]. | 2K0B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0B OCA]. | ||
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| + | ==Reference== | ||
| + | Conformation and dynamics of the three-helix bundle UBA domain of p62 from experiment and simulation., Evans CL, Long JE, Gallagher TR, Hirst JD, Searle MS, Proteins. 2007 Oct 11;71(1):227-240. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17932931 17932931] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 27 07:47:51 2008'' |
Revision as of 05:47, 27 February 2008
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NMR structure of the UBA domain of p62 (SQSTM1)
Overview
The ubiquitin associated domain of p62 is a small three-helix bundle of approximately 50 residues that mediates the recognition of polyubiquitin chains and ubiquitylated substrates. The solution structure of a 52 residue construct containing this domain has been characterized using heteronuclear nuclear magnetic resonance (NMR) methods. The resulting ensemble of NMR-derived structures was used in molecular dynamics (MD) simulations to investigate the equilibrium conformation and dynamics of this domain. NOE and (15)N relaxation data have been used to validate the structural ensemble produced by the MD simulations and show a good correlation for residues in regions of secondary structure. A similar approach was taken using an ensemble of structures from the MD simulations to calculate electronic circular dichroism (CD) and IR spectra from first principles with an encouraging correlation with the experimental CD and IR data. Proteins 2008. (c) 2007 Wiley-Liss, Inc.
About this Structure
2K0B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Conformation and dynamics of the three-helix bundle UBA domain of p62 from experiment and simulation., Evans CL, Long JE, Gallagher TR, Hirst JD, Searle MS, Proteins. 2007 Oct 11;71(1):227-240. PMID:17932931
Page seeded by OCA on Wed Feb 27 07:47:51 2008
Categories: Homo sapiens | Single protein | Cavey, J R. | Ciani, B. | Gallagher, T R.A. | Layfield, R. | Long, J E. | Searle, M S. | Sheppard, P W. | Alternative splicing | Apoptosis | Cytoplasm | Differentiation | Disease mutation | Endosome | Helical bundle | Immune response | Metal-binding | Nucleus | Phosphoprotein | Polymorphism | Signaling protein | Three helices | Ubiquitin binding | Zinc | Zinc-finger
