2dd8
From Proteopedia
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{{STRUCTURE_2dd8| PDB=2dd8 | SCENE= }} | {{STRUCTURE_2dd8| PDB=2dd8 | SCENE= }} | ||
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===Crystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing Antibody=== | ===Crystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing Antibody=== | ||
| + | {{ABSTRACT_PUBMED_16597622}} | ||
| - | + | ==Function== | |
| - | + | [[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. | |
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==About this Structure== | ==About this Structure== | ||
| - | + | [[2dd8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Sars_coronavirus Sars coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DD8 OCA]. | |
==Reference== | ==Reference== | ||
| - | <ref group="xtra">PMID: | + | <ref group="xtra">PMID:016597622</ref><references group="xtra"/><references/> |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| - | [[Category: | + | [[Category: Sars coronavirus]] |
[[Category: Dimitrov, D S.]] | [[Category: Dimitrov, D S.]] | ||
[[Category: Feng, Y.]] | [[Category: Feng, Y.]] | ||
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[[Category: Zhu, Z Y.]] | [[Category: Zhu, Z Y.]] | ||
[[Category: Antibody]] | [[Category: Antibody]] | ||
| - | [[Category: Crystal structure]] | ||
[[Category: Epitope]] | [[Category: Epitope]] | ||
| + | [[Category: Immune system-viral protein complex]] | ||
[[Category: Inhibitor]] | [[Category: Inhibitor]] | ||
[[Category: S protein]] | [[Category: S protein]] | ||
[[Category: Sar]] | [[Category: Sar]] | ||
[[Category: Vaccine]] | [[Category: Vaccine]] | ||
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:44:30 2009'' | ||
Revision as of 08:46, 23 April 2014
Contents |
Crystal Structure of SARS-CoV Spike Receptor-Binding Domain Complexed with Neutralizing Antibody
Template:ABSTRACT PUBMED 16597622
Function
[SPIKE_CVHSA] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.
About this Structure
2dd8 is a 3 chain structure with sequence from Homo sapiens and Sars coronavirus. Full crystallographic information is available from OCA.
Reference
- Prabakaran P, Gan J, Feng Y, Zhu Z, Choudhry V, Xiao X, Ji X, Dimitrov DS. Structure of severe acute respiratory syndrome coronavirus receptor-binding domain complexed with neutralizing antibody. J Biol Chem. 2006 Jun 9;281(23):15829-36. Epub 2006 Apr 5. PMID:16597622 doi:10.1074/jbc.M600697200
Categories: Homo sapiens | Sars coronavirus | Dimitrov, D S. | Feng, Y. | Gan, J H. | Ji, X. | Prabakaran, P. | Xiao, X D. | Zhu, Z Y. | Antibody | Epitope | Immune system-viral protein complex | Inhibitor | S protein | Sar | Vaccine
