This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4lrf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
{{STRUCTURE_4lrf| PDB=4lrf | SCENE= }}
+
==Phosphopentomutase S154G variant soaked with ribose 5-phosphate==
-
===Phosphopentomutase S154G variant soaked with ribose 5-phosphate===
+
<StructureSection load='4lrf' size='340' side='right' caption='[[4lrf]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
{{ABSTRACT_PUBMED_24657930}}
+
== Structural highlights ==
 +
[[4lrf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LRF OCA]. <br>
 +
<b>Related:</b> [[3un3|3un3]], [[3tx0|3tx0]], [[3twz|3twz]], [[3m8z|3m8z]], [[4lr7|4lr7]], [[4lr8|4lr8]], [[4lr9|4lr9]], [[4lra|4lra]], [[4lrb|4lrb]], [[4lrc|4lrc]], [[4lrd|4lrd]], [[4lre|4lre]]<br>
 +
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
 +
== Publication Abstract from PubMed ==
 +
Concatenation of engineered biocatalysts into multistep pathways markedly increases their utility, but the development of generalizable assembly methods remains a major challenge. Herein we evaluate 'bioretrosynthesis', which is an application of the retrograde evolution hypothesis, for biosynthetic pathway construction. To test bioretrosynthesis, we engineered a pathway for synthesis of the antiretroviral nucleoside analog didanosine (2',3'-dideoxyinosine). Applying both directed evolution- and structure-based approaches, we began pathway construction with a retro-extension from an engineered purine nucleoside phosphorylase and evolved 1,5-phosphopentomutase to accept the substrate 2,3-dideoxyribose 5-phosphate with a 700-fold change in substrate selectivity and threefold increased turnover in cell lysate. A subsequent retrograde pathway extension, via ribokinase engineering, resulted in a didanosine pathway with a 9,500-fold change in nucleoside production selectivity and 50-fold increase in didanosine production. Unexpectedly, the result of this bioretrosynthetic step was not a retro-extension from phosphopentomutase but rather the discovery of a fortuitous pathway-shortening bypass via the engineered ribokinase.
-
==Function==
+
Bioretrosynthetic construction of a didanosine biosynthetic pathway.,Birmingham WR, Starbird CA, Panosian TD, Nannemann DP, Iverson TM, Bachmann BO Nat Chem Biol. 2014 Mar 23. doi: 10.1038/nchembio.1494. PMID:24657930<ref>PMID:24657930</ref>
-
[[http://www.uniprot.org/uniprot/DEOB_BACCR DEOB_BACCR]] Phosphotransfer between the C1 and C5 carbon atoms of pentose (By similarity).
+
-
==About this Structure==
+
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-
[[4lrf]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_14579 Atcc 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LRF OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:024657930</ref><references group="xtra"/><references/>
+
</StructureSection>
[[Category: Atcc 14579]]
[[Category: Atcc 14579]]
[[Category: Phosphopentomutase]]
[[Category: Phosphopentomutase]]

Revision as of 05:24, 30 April 2014

Phosphopentomutase S154G variant soaked with ribose 5-phosphate

4lrf, resolution 2.00Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4lrf"
Personal tools