This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1wvu
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1wvu.gif|left|200px]]<br /><applet load="1wvu" size="350" color="white" frame="true" align="right" | + | [[Image:1wvu.gif|left|200px]]<br /><applet load="1wvu" size="350" color="white" frame="true" align="right" caption="1wvu, resolution 2.45Å" /> |
| - | caption="1wvu, resolution 2.45Å" /> | + | |
'''Crystal structure of chitinase C from Streptomyces griseus HUT6037'''<br /> | '''Crystal structure of chitinase C from Streptomyces griseus HUT6037'''<br /> | ||
| Line 7: | Line 6: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1WVU is a [ | + | 1WVU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_chryseus Streptomyces chryseus] with <scene name='pdbligand=CL:'>CL</scene> as [[ligand]]. Active as [http://en.wikipedia.org/wiki/Chitinase Chitinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14 3.2.1.14] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WVU OCA]. |
==Reference== | ==Reference== | ||
| - | Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:[http:// | + | Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16516924 16516924] |
[[Category: Chitinase]] | [[Category: Chitinase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 20: | ||
[[Category: whole structure]] | [[Category: whole structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Mar 18 13:26:14 2008'' |
Revision as of 11:26, 18 March 2008
|
Crystal structure of chitinase C from Streptomyces griseus HUT6037
Overview
Chitinase C (ChiC) from Streptomyces griseus HUT6037 was the first glycoside hydrolase family 19 chitinase that was found in an organism other than higher plants. An N-terminal chitin-binding domain and a C-terminal catalytic domain connected by a linker peptide constitute ChiC. We determined the crystal structure of full-length ChiC, which is the only representative of the two-domain chitinases in the family. The catalytic domain has an alpha-helix-rich fold with a deep cleft containing a catalytic site, and lacks three loops on the domain surface compared with the catalytic domain of plant chitinases. The chitin-binding domain is an all-beta protein with two tryptophan residues (Trp59 and Trp60) aligned on the surface. We suggest the binding mechanism of tri-N-acetylchitotriose onto the chitin-binding domain on the basis of molecular dynamics (MD) simulations. In this mechanism, the ligand molecule binds well on the surface-exposed binding site through two stacking interactions and two hydrogen bonds and only Trp59 and Trp60 are involved in the binding. Furthermore, the flexibility of the Trp60 side-chain, which may be involved in adjusting the binding surface to fit the surface of crystalline chitin by the rotation of chi2 angle, is shown.
About this Structure
1WVU is a Single protein structure of sequence from Streptomyces chryseus with as ligand. Active as Chitinase, with EC number 3.2.1.14 Full crystallographic information is available from OCA.
Reference
Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:16516924
Page seeded by OCA on Tue Mar 18 13:26:14 2008
