1aer

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[[Image:1aer.gif|left|200px]]<br /><applet load="1aer" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1aer.gif|left|200px]]
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caption="1aer, resolution 2.3&Aring;" />
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'''DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH BETA-TAD'''<br />
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{{Structure
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|PDB= 1aer |SIZE=350|CAPTION= <scene name='initialview01'>1aer</scene>, resolution 2.3&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=TIA:2-(1,5-DIDEOXYRIBOSE)-4-AMIDO-THIAZOLE'>TIA</scene>, <scene name='pdbligand=TAD:BETA-METHYLENE-THIAZOLE-4-CARBOXYAMIDE-ADENINE+DINUCLEOTIDE'>TAD</scene> and <scene name='pdbligand=AMP:ADENOSINE MONOPHOSPHATE'>AMP</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH BETA-TAD'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1AER is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=TIA:'>TIA</scene>, <scene name='pdbligand=TAD:'>TAD</scene> and <scene name='pdbligand=AMP:'>AMP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AER OCA].
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1AER is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AER OCA].
==Reference==
==Reference==
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Crystal structure of the catalytic domain of Pseudomonas exotoxin A complexed with a nicotinamide adenine dinucleotide analog: implications for the activation process and for ADP ribosylation., Li M, Dyda F, Benhar I, Pastan I, Davies DR, Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6902-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8692916 8692916]
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Crystal structure of the catalytic domain of Pseudomonas exotoxin A complexed with a nicotinamide adenine dinucleotide analog: implications for the activation process and for ADP ribosylation., Li M, Dyda F, Benhar I, Pastan I, Davies DR, Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6902-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8692916 8692916]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 11:43:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:56:29 2008''

Revision as of 07:56, 20 March 2008

Image:1aer.gif


PDB ID 1aer

Drag the structure with the mouse to rotate
, resolution 2.3Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH BETA-TAD


Overview

The catalytic, or third domain of Pseudomonas exotoxin A (PEIII) catalyzes the transfer of ADP ribose from nicotinamide adenine dinucleotide (NAD) to elongation factor-2 in eukaryotic cells, inhibiting protein synthesis. We have determined the structure of PEIII crystallized in the presence of NAD to define the site of binding and mechanism of activation. However, NAD undergoes a slow hydrolysis and the crystal structure revealed only the hydrolysis products, AMP and nicotinamide, bound to the enzyme. To better define the site of NAD binding, we have now crystallized PEIII in the presence of a less hydrolyzable NAD analog, beta-methylene-thiazole-4-carboxamide adenine dinucleotide (beta-TAD), and refined the complex structure at 2.3 angstroms resolution. There are two independent molecules of PEIII in the crystal, and the conformations of beta-TAD show some differences in the two binding sites. The beta-TAD attached to molecule 2 appears to have been hydrolyzed between the pyrophosphate and the nicotinamide ribose. However molecule 1 binds to an intact beta-TAD and has no crystal packing contacts in the vicinity of the binding site, so that the observed conformation and interaction with the PEIII most likely resembles that of NAD bound to PEIII in solution. We have compared this complex with the catalytic domains of diphtheria toxin, heat labile enterotoxin, and pertussis toxin, all three of which it closely resembles.

About this Structure

1AER is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Crystal structure of the catalytic domain of Pseudomonas exotoxin A complexed with a nicotinamide adenine dinucleotide analog: implications for the activation process and for ADP ribosylation., Li M, Dyda F, Benhar I, Pastan I, Davies DR, Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6902-6. PMID:8692916

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