4gji

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[[Image:4gji.jpg|left|200px]]
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==Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant H101N in complex with L-rhamnopyranose==
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<StructureSection load='4gji' size='340' side='right' caption='[[4gji]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4gji]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"achromobacter_sewerinii"_bergey_et_al._1923 "achromobacter sewerinii" bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GJI FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=RAM:ALPHA-L-RHAMNOSE'>RAM</scene>, <scene name='pdbligand=RM4:6-DEOXY-BETA-L-MANNOPYRANOSE'>RM4</scene>, <scene name='pdbligand=RNS:L-RHAMNOSE'>RNS</scene><br>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2hcv|2hcv]], [[2i56|2i56]], [[4gjj|4gjj]]</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">L-RhI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 "Achromobacter sewerinii" Bergey et al. 1923])</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-rhamnose_isomerase L-rhamnose isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.14 5.3.1.14] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gji OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gji RCSB], [http://www.ebi.ac.uk/pdbsum/4gji PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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l-Rhamnose isomerase (l-RhI) catalyzes the reversible isomerization of l-rhamnose to l-rhamnulose. Previously determined X-ray structures of l-RhI showed a hydride-shift mechanism for the isomerization of substrates in a linear form, but the mechanism for opening of the sugar-ring is still unclear. To elucidate this mechanism, we determined X-ray structures of a mutant l-RhI in complex with l-rhamnopyranose and d-allopyranose. Results suggest that a catalytic water molecule, which acts as an acid/base catalyst in the isomerization reaction, is likely to be involved in pyranose-ring opening, and that a newly found substrate sub-binding site in the vicinity of the catalytic site may recognize different anomers of substrates.
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{{STRUCTURE_4gji| PDB=4gji | SCENE= }}
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Structure of l-rhamnose isomerase in complex with l-rhamnopyranose demonstrates the sugar-ring opening mechanism and the role of a substrate sub-binding site.,Yoshida H, Yoshihara A, Teraoka M, Yamashita S, Izumori K, Kamitori S FEBS Open Bio. 2012 Dec 7;3:35-40. doi: 10.1016/j.fob.2012.11.008. Print 2013. PMID:23772372<ref>PMID:23772372</ref>
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===Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant H101N in complex with L-rhamnopyranose===
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_17141803}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[4gji]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_stutzeri Pseudomonas stutzeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GJI OCA].
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</StructureSection>
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[[Category: Achromobacter sewerinii bergey et al. 1923]]
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==Reference==
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<ref group="xtra">PMID:017141803</ref><ref group="xtra">PMID:020088877</ref><ref group="xtra">PMID:020977999</ref><references group="xtra"/>
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[[Category: L-rhamnose isomerase]]
[[Category: L-rhamnose isomerase]]
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[[Category: Pseudomonas stutzeri]]
 
[[Category: Kamitori, S.]]
[[Category: Kamitori, S.]]
[[Category: Yoshida, H.]]
[[Category: Yoshida, H.]]

Revision as of 06:56, 28 May 2014

Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant H101N in complex with L-rhamnopyranose

4gji, resolution 1.70Å

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