1ag6
From Proteopedia
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| - | [[Image:1ag6.jpg|left|200px]] | + | [[Image:1ag6.jpg|left|200px]] |
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| - | '''PLASTOCYANIN FROM SPINACH''' | + | {{Structure |
| + | |PDB= 1ag6 |SIZE=350|CAPTION= <scene name='initialview01'>1ag6</scene>, resolution 1.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CU:COPPER (II) ION'>CU</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''PLASTOCYANIN FROM SPINACH''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1AG6 is a [ | + | 1AG6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AG6 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of spinach plastocyanin at 1.7 A resolution., Xue Y, Okvist M, Hansson O, Young S, Protein Sci. 1998 Oct;7(10):2099-105. PMID:[http:// | + | Crystal structure of spinach plastocyanin at 1.7 A resolution., Xue Y, Okvist M, Hansson O, Young S, Protein Sci. 1998 Oct;7(10):2099-105. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9792096 9792096] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Spinacia oleracea]] | [[Category: Spinacia oleracea]] | ||
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[[Category: electron transport]] | [[Category: electron transport]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:57:02 2008'' |
Revision as of 07:57, 20 March 2008
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| , resolution 1.6Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PLASTOCYANIN FROM SPINACH
Overview
The crystal structure of plastocyanin from spinach has been determined using molecular replacement, with the structure of plastocyanin from poplar as a search model. Successful crystallization was facilitated by site-directed mutagenesis in which residue Gly8 was substituted with Asp. The region around residue 8 was believed to be too mobile for the wild-type protein to form crystals despite extensive screening. The current structure represents the oxidized plastocyanin, copper (II), at low pH (approximately 4.4). In contrast to the similarity in the core region as compared to its poplar counterpart, the structure shows some significant differences in loop regions. The most notable is the large shift of the 59-61 loop where the largest shift is 3.0 A for the C(alpha) atom of Glu59. This results in different patterns of electrostatic potential around the acidic patches for the two proteins.
About this Structure
1AG6 is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
Reference
Crystal structure of spinach plastocyanin at 1.7 A resolution., Xue Y, Okvist M, Hansson O, Young S, Protein Sci. 1998 Oct;7(10):2099-105. PMID:9792096
Page seeded by OCA on Thu Mar 20 09:57:02 2008
