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1b2p
From Proteopedia
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| - | [[Image:1b2p.gif|left|200px]] | + | [[Image:1b2p.gif|left|200px]] |
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| - | '''NATIVE MANNOSE-SPECIFIC BULB LECTIN FROM SCILLA CAMPANULATA (BLUEBELL) AT 1.7 ANGSTROMS RESOLUTION''' | + | {{Structure |
| + | |PDB= 1b2p |SIZE=350|CAPTION= <scene name='initialview01'>1b2p</scene>, resolution 1.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''NATIVE MANNOSE-SPECIFIC BULB LECTIN FROM SCILLA CAMPANULATA (BLUEBELL) AT 1.7 ANGSTROMS RESOLUTION''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1B2P is a [ | + | 1B2P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hyacinthoides_hispanica Hyacinthoides hispanica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1B2P OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the native (unligated) mannose-specific bulb lectin from Scilla campanulata (bluebell) at 1.7 A resolution., Wood SD, Wright LM, Reynolds CD, Rizkallah PJ, Allen AK, Peumans WJ, Van Damme EJ, Acta Crystallogr D Biol Crystallogr. 1999 Jul;55(Pt 7):1264-72. PMID:[http:// | + | Structure of the native (unligated) mannose-specific bulb lectin from Scilla campanulata (bluebell) at 1.7 A resolution., Wood SD, Wright LM, Reynolds CD, Rizkallah PJ, Allen AK, Peumans WJ, Van Damme EJ, Acta Crystallogr D Biol Crystallogr. 1999 Jul;55(Pt 7):1264-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10393293 10393293] |
[[Category: Hyacinthoides hispanica]] | [[Category: Hyacinthoides hispanica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Wright, L M.]] | [[Category: Wright, L M.]] | ||
[[Category: aglutinin]] | [[Category: aglutinin]] | ||
| - | [[Category: bluebell | + | [[Category: bluebell bulb]] |
[[Category: mannose-binding lectin]] | [[Category: mannose-binding lectin]] | ||
[[Category: monocot]] | [[Category: monocot]] | ||
| - | [[Category: protein- carbohydrate | + | [[Category: protein- carbohydrate interaction]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:05:26 2008'' |
Revision as of 08:05, 20 March 2008
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| , resolution 1.7Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
NATIVE MANNOSE-SPECIFIC BULB LECTIN FROM SCILLA CAMPANULATA (BLUEBELL) AT 1.7 ANGSTROMS RESOLUTION
Overview
The X-ray crystal structure of native Scilla campanulata agglutinin, a mannose-specific lectin from bluebell bulbs and a member of the Liliaceae family, has been determined by molecular replacement and refined to an R value of 0.186 at 1.7 A resolution. The lectin crystallizes in space group P21212 with unit-cell parameters a = 70. 42, b = 92.95, c = 46.64 A. The unit cell contains eight protein molecules of Mr = 13143 Da (119 amino-acid residues). The asymmetric unit comprises two chemically identical molecules, A and B, related by a non-crystallographic twofold axis perpendicular to c. This dimer further associates by crystallographic twofold symmetry to form a tetramer. The fold of the polypeptide backbone closely resembles that found in the lectins from Galanthus nivalis (snowdrop) and Hippeastrum (amaryllis) and contains a threefold symmetric beta-prism made up of three antiparallel four-stranded beta-sheets. Each of the four-stranded beta-sheets (I, II and III) possesses a potential saccharide-binding site containing conserved residues; however, site II has two mutations relative to sites I and III which may prevent ligation at this site. Our study provides the first accurate and detailed description of a native (unligated) structure from this superfamily of mannose-specific bulb lectins and will allow comparisons with a number of lectin-saccharide complexes which have already been determined or are currently under investigation.
About this Structure
1B2P is a Single protein structure of sequence from Hyacinthoides hispanica. Full crystallographic information is available from OCA.
Reference
Structure of the native (unligated) mannose-specific bulb lectin from Scilla campanulata (bluebell) at 1.7 A resolution., Wood SD, Wright LM, Reynolds CD, Rizkallah PJ, Allen AK, Peumans WJ, Van Damme EJ, Acta Crystallogr D Biol Crystallogr. 1999 Jul;55(Pt 7):1264-72. PMID:10393293
Page seeded by OCA on Thu Mar 20 10:05:26 2008
