1bja
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1bja.jpg|left|200px]] | + | [[Image:1bja.jpg|left|200px]] |
| - | + | ||
| - | '''ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA''' | + | {{Structure |
| + | |PDB= 1bja |SIZE=350|CAPTION= <scene name='initialview01'>1bja</scene>, resolution 2.19Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= MOTA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T4]) | ||
| + | }} | ||
| + | |||
| + | '''ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1BJA is a [ | + | 1BJA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJA OCA]. |
==Reference== | ==Reference== | ||
| - | The activation domain of the MotA transcription factor from bacteriophage T4., Finnin MS, Cicero MP, Davies C, Porter SJ, White SW, Kreuzer KN, EMBO J. 1997 Apr 15;16(8):1992-2003. PMID:[http:// | + | The activation domain of the MotA transcription factor from bacteriophage T4., Finnin MS, Cicero MP, Davies C, Porter SJ, White SW, Kreuzer KN, EMBO J. 1997 Apr 15;16(8):1992-2003. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9155025 9155025] |
[[Category: Bacteriophage t4]] | [[Category: Bacteriophage t4]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 35: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:11:46 2008'' |
Revision as of 08:11, 20 March 2008
| |||||||
| , resolution 2.19Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | MOTA (Bacteriophage T4) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ACTIVATION DOMAIN OF THE PHAGE T4 TRANSCRIPTION FACTOR MOTA
Overview
Bacteriophage T4 encodes a transcription factor, MotA, that binds to the -30 region of middle-mode promoters and activates transcription by host RNA polymerase. We have solved the structure of the MotA activation domain to 2.2 A by X-ray crystallography, and have also determined its secondary structure by NMR. An area on the surface of the protein has a distinctive patch that is populated with acidic and hydrophobic residues. Mutations within this patch cause a defective T4 growth phenotype, arguing that the patch is important for MotA function. One of the mutant MotA activation domains was purified and analyzed by NMR, and the spectra clearly show that the domain is properly folded. The mutant full-length protein appears to bind DNA normally but is deficient in transcriptional activation. We conclude that the acidic/hydrophobic surface patch is specifically involved in transcriptional activation, which is reminiscent of eukaryotic acidic activation domains.
About this Structure
1BJA is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.
Reference
The activation domain of the MotA transcription factor from bacteriophage T4., Finnin MS, Cicero MP, Davies C, Porter SJ, White SW, Kreuzer KN, EMBO J. 1997 Apr 15;16(8):1992-2003. PMID:9155025
Page seeded by OCA on Thu Mar 20 10:11:46 2008
