1c0c
From Proteopedia
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| - | [[Image:1c0c.gif|left|200px]] | + | [[Image:1c0c.gif|left|200px]] |
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| - | '''BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 4.0 DAYS''' | + | {{Structure |
| + | |PDB= 1c0c |SIZE=350|CAPTION= <scene name='initialview01'>1c0c</scene>, resolution 2.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 4.0 DAYS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1C0C is a [ | + | 1C0C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C0C OCA]. |
==Reference== | ==Reference== | ||
| - | X-ray crystal structures of a severely desiccated protein., Bell JA, Protein Sci. 1999 Oct;8(10):2033-40. PMID:[http:// | + | X-ray crystal structures of a severely desiccated protein., Bell JA, Protein Sci. 1999 Oct;8(10):2033-40. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10548049 10548049] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Pancreatic ribonuclease]] | [[Category: Pancreatic ribonuclease]] | ||
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[[Category: rna)]] | [[Category: rna)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:18:13 2008'' |
Revision as of 08:18, 20 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 4.0 DAYS
Overview
Unlike most protein crystals, form IX of bovine pancreatic ribonuclease A diffracts well when severely dehydrated. Crystal structures have been solved after 2.5 and 4 days of desiccation with CaSO4, at 1.9 and 2.0 A resolution, respectively. The two desiccated structures are very similar. An RMS displacement of 1.6 A is observed for main-chain atoms in each structure when compared to the hydrated crystal structure with some large rearrangements observed in loop regions. The structural changes are the result of intermolecular contacts formed by strong electrostatic interactions in the absence of a high dielectric medium. The electron density is very diffuse for some surface loops, consistent with a very disordered structure. This disorder is related to the conformational changes. These results help explain conformational changes during the lyophilization of protein and the associated phenomena of denaturation and molecular memory.
About this Structure
1C0C is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
X-ray crystal structures of a severely desiccated protein., Bell JA, Protein Sci. 1999 Oct;8(10):2033-40. PMID:10548049
Page seeded by OCA on Thu Mar 20 10:18:13 2008
