This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1c4e
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1c4e.jpg|left|200px]] | + | [[Image:1c4e.jpg|left|200px]] |
| - | + | ||
| - | '''GURMARIN FROM GYMNEMA SYLVESTRE''' | + | {{Structure |
| + | |PDB= 1c4e |SIZE=350|CAPTION= <scene name='initialview01'>1c4e</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''GURMARIN FROM GYMNEMA SYLVESTRE''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1C4E is a [ | + | 1C4E is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gymnema_sylvestre Gymnema sylvestre]. This structure supersedes the now removed PDB entry 2GUR. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C4E OCA]. |
==Reference== | ==Reference== | ||
| - | High-resolution solution structure of gurmarin, a sweet-taste-suppressing plant polypeptide., Fletcher JI, Dingley AJ, Smith R, Connor M, Christie MJ, King GF, Eur J Biochem. 1999 Sep;264(2):525-33. PMID:[http:// | + | High-resolution solution structure of gurmarin, a sweet-taste-suppressing plant polypeptide., Fletcher JI, Dingley AJ, Smith R, Connor M, Christie MJ, King GF, Eur J Biochem. 1999 Sep;264(2):525-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10491100 10491100] |
[[Category: Gymnema sylvestre]] | [[Category: Gymnema sylvestre]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: sweet taste transduction]] | [[Category: sweet taste transduction]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:19:40 2008'' |
Revision as of 08:19, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
GURMARIN FROM GYMNEMA SYLVESTRE
Overview
Gurmarin is a 35-residue polypeptide from the Asclepiad vine Gymnema sylvestre. It has been utilised as a pharmacological tool in the study of sweet-taste transduction because of its ability to selectively inhibit the neural response to sweet tastants in rats. We have chemically synthesised and folded gurmarin and determined its three-dimensional solution structure to high resolution using two-dimensional NMR spectroscopy. Structure calculations utilised 612 interproton-distance, 19 dihedral-angle, and 18 hydrogen-bond restraints. The structure is well defined for residues 3-34, with backbone and heavy atom rms differences of 0.27 +/- 0.09 A and 0.73 +/- 0.09 A, respectively. Gurmarin adopts a compact structure containing an antiparallel beta-hairpin (residues 22-34), several well-defined beta-turns, and a cystine-knot motif commonly observed in toxic and inhibitory polypeptides. Despite striking structural homology with delta-atracotoxin, a spider neurotoxin known to slow the inactivation of voltage-gated Na+ channels, we show that gurmarin has no effect on a variety of voltage-sensitive channels.
About this Structure
1C4E is a Single protein structure of sequence from Gymnema sylvestre. This structure supersedes the now removed PDB entry 2GUR. Full crystallographic information is available from OCA.
Reference
High-resolution solution structure of gurmarin, a sweet-taste-suppressing plant polypeptide., Fletcher JI, Dingley AJ, Smith R, Connor M, Christie MJ, King GF, Eur J Biochem. 1999 Sep;264(2):525-33. PMID:10491100
Page seeded by OCA on Thu Mar 20 10:19:40 2008
