1d8z
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1d8z.gif|left|200px]] | + | [[Image:1d8z.gif|left|200px]] |
| - | + | ||
| - | '''SOLUTION STRUCTURE OF THE FIRST RNA-BINDING DOMAIN (RBD1) OF HU ANTIGEN C (HUC)''' | + | {{Structure |
| + | |PDB= 1d8z |SIZE=350|CAPTION= <scene name='initialview01'>1d8z</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''SOLUTION STRUCTURE OF THE FIRST RNA-BINDING DOMAIN (RBD1) OF HU ANTIGEN C (HUC)''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1D8Z is a [ | + | 1D8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8Z OCA]. |
==Reference== | ==Reference== | ||
| - | NMR studies on functional structures of the AU-rich element-binding domains of Hu antigen C., Inoue M, Muto Y, Sakamoto H, Yokoyama S, Nucleic Acids Res. 2000 Apr 15;28(8):1743-50. PMID:[http:// | + | NMR studies on functional structures of the AU-rich element-binding domains of Hu antigen C., Inoue M, Muto Y, Sakamoto H, Yokoyama S, Nucleic Acids Res. 2000 Apr 15;28(8):1743-50. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10734193 10734193] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: rna-binding domain]] | [[Category: rna-binding domain]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:34:49 2008'' |
Revision as of 08:34, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE FIRST RNA-BINDING DOMAIN (RBD1) OF HU ANTIGEN C (HUC)
Overview
Hu antigen C (HuC) has three RNA-binding domains (RBDs). The N-terminal two, RBD1 and RBD2, are linked in tandem and bind to the AU-rich elements (AREs) in the 3'-untranslated region of particular mRNAs. The solution structures of HuC RBD1 and RBD2 were determined by NMR methods. The HuC RBD1 and RBD2 structures are quite similar to those of Sxl RBD1 and RBD2, respectively. The individual RBDs of HuC, RBD1 and RBD2 in isolation can interact rather weakly with the minimal ARE motif, AUUUA, while the didomain fragment, RBD1-RBD2, of HuC binds more tightly to a longer ARE RNA, UAUUUAUUUU. Chemical shift perturbations by the longer RNA on HuC RBD1-RBD2 were mapped on and around the two beta-sheets and on the C-terminal region of RBD1. The HuC RBD1-RBD2 residues that exhibited significant chemical shift perturbations coincide with those conserved in Sxl RBD1-RBD2. These data indicate that the RNA-binding characteristics of the HuC and Sxl didomain fragments are similar, even though the target RNAs and the biological functions of the proteins are different.
About this Structure
1D8Z is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
NMR studies on functional structures of the AU-rich element-binding domains of Hu antigen C., Inoue M, Muto Y, Sakamoto H, Yokoyama S, Nucleic Acids Res. 2000 Apr 15;28(8):1743-50. PMID:10734193
Page seeded by OCA on Thu Mar 20 10:34:49 2008
