1dbn

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[[Image:1dbn.gif|left|200px]]<br /><applet load="1dbn" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1dbn.gif|left|200px]]
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caption="1dbn, resolution 2.75&Aring;" />
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'''MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE'''<br />
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{{Structure
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|PDB= 1dbn |SIZE=350|CAPTION= <scene name='initialview01'>1dbn</scene>, resolution 2.75&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1DBN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA].
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1DBN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Maackia_amurensis Maackia amurensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBN OCA].
==Reference==
==Reference==
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An unusual carbohydrate binding site revealed by the structures of two Maackia amurensis lectins complexed with sialic acid-containing oligosaccharides., Imberty A, Gautier C, Lescar J, Perez S, Wyns L, Loris R, J Biol Chem. 2000 Jun 9;275(23):17541-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10747930 10747930]
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An unusual carbohydrate binding site revealed by the structures of two Maackia amurensis lectins complexed with sialic acid-containing oligosaccharides., Imberty A, Gautier C, Lescar J, Perez S, Wyns L, Loris R, J Biol Chem. 2000 Jun 9;275(23):17541-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10747930 10747930]
[[Category: Maackia amurensis]]
[[Category: Maackia amurensis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: sialyllactose]]
[[Category: sialyllactose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:35:50 2008''

Revision as of 08:35, 20 March 2008

Image:1dbn.gif


PDB ID 1dbn

Drag the structure with the mouse to rotate
, resolution 2.75Å
Ligands: , and
Coordinates: save as pdb, mmCIF, xml



MAACKIA AMURENSIS LEUKOAGGLUTININ (LECTIN) WITH SIALYLLACTOSE


Overview

Seeds from the legume tree Maackia amurensis contain two lectins that can agglutinate different blood cell types. Their specificity toward sialylated oligosaccharides is unique among legume lectins; the leukoagglutinin preferentially binds to sialyllactosamine (alphaNeuAc(2-3)betaGal(1-4)betaGlcNAc), whereas the hemagglutinin displays higher affinity for a disialylated tetrasaccharide (alphaNeuAc(2-3)betaGal(1-3)[alphaNeuAc(2-6)]alphaG alNAc). The three-dimensional structure of the complex between M. amurensis leukoagglutinin and sialyllactose has been determined at 2.75-A resolution using x-ray crystallography. The carbohydrate binding site consists of a deep cleft that accommodates the three carbohydrate residues of the sialyllactose. The central galactose sits in the primary binding site in an orientation that has not been observed previously in other legume lectins. The carboxyl group of sialic acid establishes a salt bridge with a lysine side chain. The glucose residue is very efficiently docked between two tyrosine aromatic rings. The complex between M. amurensis hemagglutinin and a disialylated tetrasaccharide could be modeled from the leukoagglutinin/sialyllactose crystal structure. The substitution of one tyrosine by an alanine residue is responsible for the difference in fine specificity between the two isolectins. Comparison with other legume lectins indicates that oligosaccharide specificity within this family is achieved by the recycling of structural loops in different combinations.

About this Structure

1DBN is a Single protein structure of sequence from Maackia amurensis. Full crystallographic information is available from OCA.

Reference

An unusual carbohydrate binding site revealed by the structures of two Maackia amurensis lectins complexed with sialic acid-containing oligosaccharides., Imberty A, Gautier C, Lescar J, Perez S, Wyns L, Loris R, J Biol Chem. 2000 Jun 9;275(23):17541-8. PMID:10747930

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