Ectonucleoside triphosphate diphosphohydrolase

Function

Ectonucleoside triphosphate diphosphohydrolase (NTPDase) hydrolyzes nucleoside 5’-triphosphate to nucleoside 5’-phosphate and 2 phosphates. NTPDase can hydrolyze ATP and ADP. NTPDase has an important role in regulating neutrophil chemotaxis. NTPDase 1,2,3,8 are cell surface enzymes with catalytic sites facing extracellularly while other NTPDases act intracellularly. NTPDase require Ca+2 or Mg+2 for their activity. The NTPDases can be differentiated according to their substrate specificity, divalent cation usage and product formation.

Disease

Relevance

Structural highlights

3D structures of ectonucleoside triphosphate diphosphohydrolase

Updated on 11-August-2014

NTPDase1

3aap – LpNTPDase – Legionella pneumophila
3aaq – LpNTPDase + inhibitor
3aar – LpNTPDase + AMPPNP
3zx3 – rNTPDase extracellular domain - rat
3zx0, 3zx2 – rNTPDase extracellular domain + polyoxometallates
4jep – TgNTPDase – Toxoplasma gondii
4a5b – TgNTPDase (mutant)
4kh6 – TgNTPDase (mutant) + adenosine-imido-diphosphate

NTPDase1 open form

4br4, 4brm – LpNTPDase
4br7, 4brc – LpNTPDase + adenosine-imido-diphosphate

NTPDase1 closed form

4br9 – LpNTPDase
4bra – LpNTPDase + AMPPNP
4brc – LpNTPDase + adenosine-imido-diphosphate
4brd – LpNTPDase (mutant) + AMPPNP
4bre – LpNTPDase + adenosine-5’-phosphovanadate
4brh – LpNTPDase + thyamine-5’-phosphovanadate
4brl – LpNTPDase + guanosine-5’-phosphovanadate
4brf, 4brn – LpNTPDase + AMP
4brg – LpNTPDase + GMPPNP
4bri – LpNTPDase + UMPPNP
4brk – LpNTPDase (mutant) + UMPPNP
4brq – LpNTPDase + AMP + phosphate

NTPDase1 part-open form

4bro – LpNTPDase
4bvp, 4bvo – LpNTPDase + polyoxometallates

NTPDase2

3cj1 – rNTPDase extracellular domain
3cj7 – rNTPDase extracellular domain + AMP
3cj9 – rNTPDase extracellular domain + AMP + phosphate
3cja – rNTPDase extracellular domain + AMPPNP
4bqz – rNTPDase extracellular domain + GMPPNP
4br2 – rNTPDase extracellular domain + UMPPNP
4br0 – rNTPDase extracellular domain + adenosine-imido-diphosphate
4cd1 – rNTPDase extracellular domain + inhibitor
4cd3 – rNTPDase extracellular domain (mutant) + inhibitor

NTPDase3

4a57 – TgNTPDase
4a59 – TgNTPDase + AMP
4a5a, 4kh4 – TgNTPDase (mutant) + AMPPNP
4kh5 – TgNTPDase (mutant) + adenosine-imido-diphosphate

References