1e3m
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1e3m.gif|left|200px]] | + | [[Image:1e3m.gif|left|200px]] |
| - | + | ||
| - | '''THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:T MISMATCH''' | + | {{Structure |
| + | |PDB= 1e3m |SIZE=350|CAPTION= <scene name='initialview01'>1e3m</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= MUTS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | }} | ||
| + | |||
| + | '''THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:T MISMATCH''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1E3M is a [ | + | 1E3M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3M OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch., Lamers MH, Perrakis A, Enzlin JH, Winterwerp HH, de Wind N, Sixma TK, Nature. 2000 Oct 12;407(6805):711-7. PMID:[http:// | + | The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch., Lamers MH, Perrakis A, Enzlin JH, Winterwerp HH, de Wind N, Sixma TK, Nature. 2000 Oct 12;407(6805):711-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11048711 11048711] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 24: | Line 33: | ||
[[Category: mismatch recognition]] | [[Category: mismatch recognition]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:49:08 2008'' |
Revision as of 08:49, 20 March 2008
| |||||||
| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | MUTS (Escherichia coli) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH A G:T MISMATCH
Overview
DNA mismatch repair ensures genomic integrity on DNA replication. Recognition of a DNA mismatch by a dimeric MutS protein initiates a cascade of reactions and results in repair of the newly synthesized strand; however, details of the molecular mechanism remain controversial. Here we present the crystal structure at 2.2 A of MutS from Escherichia coli bound to a G x T mismatch. The two MutS monomers have different conformations and form a heterodimer at the structural level. Only one monomer recognizes the mismatch specifically and has ADP bound. Mismatch recognition occurs by extensive minor groove interactions causing unusual base pairing and kinking of the DNA. Nonspecific major groove DNA-binding domains from both monomers embrace the DNA in a clamp-like structure. The interleaved nucleotide-binding sites are located far from the DNA. Mutations in human MutS alpha (MSH2/MSH6) that lead to hereditary predisposition for cancer, such as hereditary non-polyposis colorectal cancer, can be mapped to this crystal structure.
About this Structure
1E3M is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The crystal structure of DNA mismatch repair protein MutS binding to a G x T mismatch., Lamers MH, Perrakis A, Enzlin JH, Winterwerp HH, de Wind N, Sixma TK, Nature. 2000 Oct 12;407(6805):711-7. PMID:11048711
Page seeded by OCA on Thu Mar 20 10:49:08 2008
