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1e3t
From Proteopedia
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| - | [[Image:1e3t.jpg|left|200px]]< | + | [[Image:1e3t.jpg|left|200px]] |
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| - | '''SOLUTION STRUCTURE OF THE NADP(H) BINDING COMPONENT (DIII) OF PROTON-TRANSLOCATING TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM''' | + | {{Structure |
| + | |PDB= 1e3t |SIZE=350|CAPTION= <scene name='initialview01'>1e3t</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAP:NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NAP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/NAD(P)(+)_transhydrogenase_(B-specific) NAD(P)(+) transhydrogenase (B-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.1.1 1.6.1.1] | ||
| + | |GENE= PNTB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1085 Rhodospirillum rubrum]) | ||
| + | }} | ||
| + | |||
| + | '''SOLUTION STRUCTURE OF THE NADP(H) BINDING COMPONENT (DIII) OF PROTON-TRANSLOCATING TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1E3T is a [ | + | 1E3T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3T OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum., Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB, Biochim Biophys Acta. 2000 Aug 15;1459(2-3):248-57. PMID:[http:// | + | Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum., Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB, Biochim Biophys Acta. 2000 Aug 15;1459(2-3):248-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11004437 11004437] |
[[Category: NAD(P)(+) transhydrogenase (B-specific)]] | [[Category: NAD(P)(+) transhydrogenase (B-specific)]] | ||
[[Category: Rhodospirillum rubrum]] | [[Category: Rhodospirillum rubrum]] | ||
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[[Category: transhydrogenase]] | [[Category: transhydrogenase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:49:09 2008'' |
Revision as of 08:49, 20 March 2008
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| Ligands: | |||||||
| Gene: | PNTB (Rhodospirillum rubrum) | ||||||
| Activity: | NAD(P)(+) transhydrogenase (B-specific), with EC number 1.6.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF THE NADP(H) BINDING COMPONENT (DIII) OF PROTON-TRANSLOCATING TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM
Overview
Transhydrogenase is a proton pump found in the membranes of bacteria and animal mitochondria. The solution structure of the expressed, 21.5 kDa, NADP(H)-binding component (dIII) of transhydrogenase from Rhodospirillum rubrum has been solved by NMR methods. This is the first description of the structure of dIII from a bacterial source. The protein adopts a Rossmann fold: an open, twisted, parallel beta-sheet, flanked by helices. However, the binding of NADP(+) to dIII is profoundly different to that seen in other Rossmann structures, in that its orientation is reversed: the adenosine moiety interacts with the first betaalphabetaalphabeta motif, and the nicotinamide with the second. Features in the structure that might be responsible for changes in nucleotide-binding affinity during catalysis, and for interaction with other components of the enzyme, are identified. The results are compared with the recently determined, high-resolution crystal structures of human and bovine dIII which also show the reversed nucleotide orientation.
About this Structure
1E3T is a Single protein structure of sequence from Rhodospirillum rubrum. Full crystallographic information is available from OCA.
Reference
Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum., Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB, Biochim Biophys Acta. 2000 Aug 15;1459(2-3):248-57. PMID:11004437
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