1e5h

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[[Image:1e5h.jpg|left|200px]]<br /><applet load="1e5h" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1e5h.jpg|left|200px]]
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caption="1e5h, resolution 1.96&Aring;" />
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'''DELTA-R307A DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE AND CARBON DIOXIDE'''<br />
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{{Structure
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|PDB= 1e5h |SIZE=350|CAPTION= <scene name='initialview01'>1e5h</scene>, resolution 1.96&Aring;
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|SITE= <scene name='pdbsite=FE:Succinate+Binding+Site'>FE</scene>
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|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene> and <scene name='pdbligand=CO2:CARBON DIOXIDE'>CO2</scene>
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|ACTIVITY=
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|GENE= CEFE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 Streptomyces clavuligerus])
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}}
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'''DELTA-R307A DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE AND CARBON DIOXIDE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1E5H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus] with <scene name='pdbligand=FE2:'>FE2</scene>, <scene name='pdbligand=SIN:'>SIN</scene> and <scene name='pdbligand=CO2:'>CO2</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=FE:Succinate+Binding+Site'>FE</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5H OCA].
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1E5H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5H OCA].
==Reference==
==Reference==
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Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)., Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ, J Mol Biol. 2001 May 18;308(5):937-48. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11352583 11352583]
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Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)., Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ, J Mol Biol. 2001 May 18;308(5):937-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11352583 11352583]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptomyces clavuligerus]]
[[Category: Streptomyces clavuligerus]]
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[[Category: SIN]]
[[Category: SIN]]
[[Category: 2-oxoglutarate]]
[[Category: 2-oxoglutarate]]
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[[Category: c-terminus antibiotics]]
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[[Category: c-terminus antibiotic]]
[[Category: cephalosporin]]
[[Category: cephalosporin]]
[[Category: ferrous oxygenase]]
[[Category: ferrous oxygenase]]
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[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:24:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:50:04 2008''

Revision as of 08:50, 20 March 2008

Image:1e5h.jpg


PDB ID 1e5h

Drag the structure with the mouse to rotate
, resolution 1.96Å
Sites:
Ligands: , and
Gene: CEFE (Streptomyces clavuligerus)
Coordinates: save as pdb, mmCIF, xml



DELTA-R307A DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH SUCCINATE AND CARBON DIOXIDE


Overview

Deacetoxycephalosporin C synthase (DAOCS) is an iron(II) and 2-oxoglutarate-dependent oxygenase that catalyzes the conversion of penicillin N to deacetoxycephalosporin C, the committed step in the biosynthesis of cephalosporin antibiotics. The crystal structure of DAOCS revealed that the C terminus of one molecule is inserted into the active site of its neighbor in a cyclical fashion within a trimeric unit. This arrangement has hindered the generation of crystalline enzyme-substrate complexes. Therefore, we constructed a series of DAOCS mutants with modified C termini. Oxidation of 2-oxoglutarate was significantly uncoupled from oxidation of the penicillin substrate in certain truncated mutants. The extent of uncoupling varied with the number of residues deleted and the penicillin substrate used. Crystal structures were determined for the DeltaR306 mutant complexed with iron(II) and 2-oxoglutarate (to 2.10 A) and the DeltaR306A mutant complexed with iron(II), succinate and unhydrated carbon dioxide (to 1.96 A). The latter may mimic a product complex, and supports proposals for a metal-bound CO(2) intermediate during catalysis.

About this Structure

1E5H is a Single protein structure of sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.

Reference

Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)., Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ, J Mol Biol. 2001 May 18;308(5):937-48. PMID:11352583

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