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4p7b
From Proteopedia
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p7b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p7b RCSB], [http://www.ebi.ac.uk/pdbsum/4p7b PDBsum]</span></td></tr> | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4p7b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4p7b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4p7b RCSB], [http://www.ebi.ac.uk/pdbsum/4p7b PDBsum]</span></td></tr> | ||
<table> | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Peptidyl-tRNA hydrolase (Pth; EC 3.1.1.29) from the pathogenic bacterium Salmonella typhimurium has been cloned, expressed in Escherichia coli and crystallized for X-ray analysis. Crystals were grown using hanging-drop vapor diffusion against a reservoir solution consisting of 0.03 M citric acid, 0.05 M bis-tris propane, 1% glycerol, 3% sucrose, 25% PEG 6000 pH 7.6. Crystals were used to obtain the three-dimensional structure of the native protein at 1.6 A resolution. The structure was determined by molecular replacement of the crystallographic data processed in space group P2(1)2(1)2(1) with unit-cell parameters a=62.1, b=64.9, c=110.5 A, alpha=beta=gamma=90 degrees . The asymmetric unit of the crystallographic lattice was composed of two copies of the enzyme molecule with a 51% solvent fraction, corresponding to a Matthews coefficient of 2.02 A3 Da(-1). The structural coordinates reported serve as a foundation for computational and structure-guided efforts towards novel small-molecule Pth1 inhibitors and potential antibacterial development. | ||
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| + | Recombinant production, crystallization and X-ray crystallographic structure determination of peptidyl-tRNA hydrolase from Salmonella typhimurium.,Vandavasi V, Taylor-Creel K, McFeeters RL, Coates L, McFeeters H Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):872-7. doi:, 10.1107/S2053230X14009893. Epub 2014 Jun 18. PMID:25005080<ref>PMID:25005080</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 07:02, 24 September 2014
Crystal structure of S. typhimurium peptidyl-tRNA hydrolase
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