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1eq1

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[[Image:1eq1.gif|left|200px]]<br /><applet load="1eq1" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1eq1.gif|left|200px]]
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caption="1eq1" />
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'''NMR STRUCTURE OF AN EXCHANGEABLE APOLIPOPROTEIN-MANDUCA SEXTA APOLIPOPHORIN-III'''<br />
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{{Structure
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|PDB= 1eq1 |SIZE=350|CAPTION= <scene name='initialview01'>1eq1</scene>
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|SITE=
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|LIGAND=
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|ACTIVITY=
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|GENE=
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}}
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'''NMR STRUCTURE OF AN EXCHANGEABLE APOLIPOPROTEIN-MANDUCA SEXTA APOLIPOPHORIN-III'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1EQ1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQ1 OCA].
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1EQ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Manduca_sexta Manduca sexta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQ1 OCA].
==Reference==
==Reference==
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Structural basis for the conformational adaptability of apolipophorin III, a helix-bundle exchangeable apolipoprotein., Wang J, Sykes BD, Ryan RO, Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1188-93. Epub 2002 Jan 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11818551 11818551]
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Structural basis for the conformational adaptability of apolipophorin III, a helix-bundle exchangeable apolipoprotein., Wang J, Sykes BD, Ryan RO, Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1188-93. Epub 2002 Jan 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11818551 11818551]
[[Category: Manduca sexta]]
[[Category: Manduca sexta]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Sykes, B D.]]
[[Category: Sykes, B D.]]
[[Category: Wang, J.]]
[[Category: Wang, J.]]
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[[Category: "helix-short helix-helix" recognition motif]]
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[[Category: helix-short helix-helix recognition motif]]
[[Category: five helix-bundle]]
[[Category: five helix-bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:30:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:58:55 2008''

Revision as of 08:58, 20 March 2008


PDB ID 1eq1

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NMR STRUCTURE OF AN EXCHANGEABLE APOLIPOPROTEIN-MANDUCA SEXTA APOLIPOPHORIN-III


Overview

The high-resolution NMR structure of apolipophorin III from the sphinx moth, Manduca sexta, has been determined in the lipid-free state. We show that lipid-free apolipophorin III adopts a unique helix-bundle topology that has several characteristic structural features. These include a marginally stable, up-and-down helix bundle that allows for concerted opening of the bundle about "hinged" loops upon lipid interaction and buried polar/ionizable residues and buried interhelical H-bonds located in the otherwise hydrophobic interior of the bundle that adjust protein stability and facilitate lipid-induced conformational opening. We suggest that these structural features modulate the conformational adaptability of the lipid-free helix bundle upon lipid binding and control return of the open conformation to the original lipid-free helix-bundle state. Taken together, these data provide a structural rationale for the ability of exchangeable apolipoproteins to reversibly interact with circulating lipoprotein particles.

About this Structure

1EQ1 is a Single protein structure of sequence from Manduca sexta. Full crystallographic information is available from OCA.

Reference

Structural basis for the conformational adaptability of apolipophorin III, a helix-bundle exchangeable apolipoprotein., Wang J, Sykes BD, Ryan RO, Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1188-93. Epub 2002 Jan 29. PMID:11818551

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