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1eyx

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[[Image:1eyx.gif|left|200px]]<br /><applet load="1eyx" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1eyx.gif|left|200px]]
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caption="1eyx, resolution 2.25&Aring;" />
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'''CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS'''<br />
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{{Structure
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|PDB= 1eyx |SIZE=350|CAPTION= <scene name='initialview01'>1eyx</scene>, resolution 2.25&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene> and <scene name='pdbligand=PUB:PHYCOUROBILIN'>PUB</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1EYX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gracilaria_chilensis Gracilaria chilensis] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=BLA:'>BLA</scene>, <scene name='pdbligand=CYC:'>CYC</scene> and <scene name='pdbligand=PUB:'>PUB</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYX OCA].
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1EYX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gracilaria_chilensis Gracilaria chilensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYX OCA].
==Reference==
==Reference==
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Crystallization and 2.2 A resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning., Contreras-Martel C, Martinez-Oyanedel J, Bunster M, Legrand P, Piras C, Vernede X, Fontecilla-Camps JC, Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):52-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11134927 11134927]
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Crystallization and 2.2 A resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning., Contreras-Martel C, Martinez-Oyanedel J, Bunster M, Legrand P, Piras C, Vernede X, Fontecilla-Camps JC, Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):52-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11134927 11134927]
[[Category: Gracilaria chilensis]]
[[Category: Gracilaria chilensis]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: r-phycoerythrin]]
[[Category: r-phycoerythrin]]
[[Category: red algae]]
[[Category: red algae]]
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[[Category: sequences]]
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[[Category: sequence]]
[[Category: twin]]
[[Category: twin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:32:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:02:08 2008''

Revision as of 09:02, 20 March 2008

Image:1eyx.gif


PDB ID 1eyx

Drag the structure with the mouse to rotate
, resolution 2.25Å
Ligands: , , and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF R-PHYCOERYTHRIN AT 2.2 ANGSTROMS


Overview

R-phycoerythrin, a light-harvesting component from the red algae Gracilaria chilensis, was crystallized by vapour diffusion using ammonium sulfate as precipitant agent. Red crystals grew after one week at 293 K and diffracted to 2.70 A resolution. Three serial macroseeding assays were necessary to grow a second larger crystal to dimensions of 0.68 x 0.16 x 0.16 mm. This crystal diffracted to 2.24 A resolution using synchrotron radiation at beamline BM14 of the European Synchrotron Radiation Facility (ESRF) at Grenoble, France and was used for structure determination. Data were collected at 100 K to a completeness of 98.6%. The crystal was trigonal, space group R3, with unit-cell parameters a = b = 187.3, c = 59.1 A, alpha = beta = 90, gamma = 120 degrees. Data treatment using the CCP4 suite of programs indicated that the crystal was twinned ((I(2))/(I)(2) = 1.41). Molecular replacement was performed with AMoRe using the R-phycoerythrin from Polysiphonia urceolata [Chang et al. (1996), J. Mol. Biol. 249, 424-440] as a search model. In order to overcome the twinning problem, SHELX97 was used for the crystallographic refinement. The twin fraction was 0.48, indicating a nearly perfect hemihedrally twinned crystal. The final R(work) and R(free) factors are 0.16 and 0.25, respectively. All the residues and chromophores of the alpha- and beta-chains are well defined in the electron-density maps. Some residues belonging to the gamma-linker are also recognizable.

About this Structure

1EYX is a Protein complex structure of sequences from Gracilaria chilensis. Full crystallographic information is available from OCA.

Reference

Crystallization and 2.2 A resolution structure of R-phycoerythrin from Gracilaria chilensis: a case of perfect hemihedral twinning., Contreras-Martel C, Martinez-Oyanedel J, Bunster M, Legrand P, Piras C, Vernede X, Fontecilla-Camps JC, Acta Crystallogr D Biol Crystallogr. 2001 Jan;57(Pt 1):52-60. PMID:11134927

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