1rtc

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[[Image:1rtc.png|left|200px]]
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==THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS==
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<StructureSection load='1rtc' size='340' side='right' caption='[[1rtc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rtc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RTC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RTC FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rtc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rtc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rtc RCSB], [http://www.ebi.ac.uk/pdbsum/1rtc PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rt/1rtc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The plant cytotoxin ricin is a heterodimer with a cell surface binding (B) chain and an enzymatically active A chain (RTA) known to act as a specific N-glycosidase. RTA must be separated from B chain to attack rRNA. The X-ray structure of ricin has been solved recently; here we report the structure of the isolated A chain expressed from a clone in Escherichia coli. This structure of wild-type rRTA has and will continue to serve as the parent compound for difference Fouriers used to assess the structure of site-directed mutants designed to analyze the mechanism of this medically and commercially important toxin. The structure of the recombinant protein, rRTA, is virtually identical to that seen previously for A chain in the heterodimeric toxin. Some minor conformational changes due to interactions with B chain and to crystal packing differences are described. Perhaps the most significant difference is the presence in rRTA of an additional active site water. This molecule is positioned to act as the ultimate nucleophile in the depurination reaction mechanism proposed by Monzingo and Robertus (1992, J. Mol. Biol. 227, 1136-1145).
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{{STRUCTURE_1rtc| PDB=1rtc | SCENE= }}
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Structure of recombinant ricin A chain at 2.3 A.,Mlsna D, Monzingo AF, Katzin BJ, Ernst S, Robertus JD Protein Sci. 1993 Mar;2(3):429-35. PMID:8453380<ref>PMID:8453380</ref>
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===THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_8453380}}
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==About this Structure==
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[[1rtc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ricinus_communis Ricinus communis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RTC OCA].
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==See Also==
==See Also==
*[[Ricin|Ricin]]
*[[Ricin|Ricin]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:008453380</ref><ref group="xtra">PMID:009115985</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Ricinus communis]]
[[Category: Ricinus communis]]
[[Category: RRNA N-glycosylase]]
[[Category: RRNA N-glycosylase]]

Revision as of 21:59, 28 September 2014

THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS

1rtc, resolution 2.30Å

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