1fyu
From Proteopedia
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| - | [[Image:1fyu.gif|left|200px]] | + | [[Image:1fyu.gif|left|200px]] |
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| - | '''Crystal structure of erythrina corallodendron lectin in hexagonal crystal form''' | + | {{Structure |
| + | |PDB= 1fyu |SIZE=350|CAPTION= <scene name='initialview01'>1fyu</scene>, resolution 2.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of erythrina corallodendron lectin in hexagonal crystal form''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FYU is a [ | + | 1FYU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Erythrina_corallodendron Erythrina corallodendron]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FYU OCA]. |
==Reference== | ==Reference== | ||
| - | Chemical characteristics of dimer interfaces in the legume lectin family., Elgavish S, Shaanan B, Protein Sci. 2001 Apr;10(4):753-61. PMID:[http:// | + | Chemical characteristics of dimer interfaces in the legume lectin family., Elgavish S, Shaanan B, Protein Sci. 2001 Apr;10(4):753-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11274466 11274466] |
[[Category: Erythrina corallodendron]] | [[Category: Erythrina corallodendron]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-carbohydrate complex]] | [[Category: protein-carbohydrate complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:15:47 2008'' |
Revision as of 09:15, 20 March 2008
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| , resolution 2.60Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of erythrina corallodendron lectin in hexagonal crystal form
Overview
The Erythrina corallodendron lectin (EcorL) crystallizes in monoclinic and hexagonal crystal forms. Comparison of the newly determined hexagonal form (PDB code 1fyu) with the monoclinic form shows that the dimeric structure of EcorL reflects the inherent biological structure of the protein and is not an artifact of the crystal packing. To further understand the factors determining the dimerization modes of legume lectins, EcorL, concanavalin A (ConA), and Griffonia simplicifolia (GS4) were taken as representatives of the three unique dimers found in the family. Six virtual homodimers were generated. The hydropathy, amino acid composition, and solvation energy were calculated for all nine homodimers. Each of the three native dimers has a distinct chemical composition. EcorL has a dominant hydrophobic component, and ConA has a strong polar component, but in GS4 the three components contribute equally to the interface. This distribution pattern at the interface is unique to the native dimers and distinct from the partition observed in the virtual dimers. Amino acid composition of other members of the family that dimerize like EcorL or ConA maintain the same pattern of amino acids distribution observed in EcorL and ConA. However, lectins that dimerize like GS4 do not show a particularly distinct distribution. In all cases, the calculated solvation energy of the native dimer was lower than that of the virtual dimers, suggesting that the observed mode of dimerization is the most stable organization for the given sequence and tertiary structure. The dimerization type cannot be predicted by sequence analysis.
About this Structure
1FYU is a Single protein structure of sequence from Erythrina corallodendron. Full crystallographic information is available from OCA.
Reference
Chemical characteristics of dimer interfaces in the legume lectin family., Elgavish S, Shaanan B, Protein Sci. 2001 Apr;10(4):753-61. PMID:11274466
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