1g2d
From Proteopedia
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| - | [[Image:1g2d.gif|left|200px]] | + | [[Image:1g2d.gif|left|200px]] |
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| - | '''STRUCTURE OF A CYS2HIS2 ZINC FINGER/TATA BOX COMPLEX (CLONE #2)''' | + | {{Structure |
| + | |PDB= 1g2d |SIZE=350|CAPTION= <scene name='initialview01'>1g2d</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF A CYS2HIS2 ZINC FINGER/TATA BOX COMPLEX (CLONE #2)''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1G2D is a [ | + | 1G2D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G2D OCA]. |
==Reference== | ==Reference== | ||
| - | Beyond the "recognition code": structures of two Cys2His2 zinc finger/TATA box complexes., Wolfe SA, Grant RA, Elrod-Erickson M, Pabo CO, Structure. 2001 Aug;9(8):717-23. PMID:[http:// | + | Beyond the "recognition code": structures of two Cys2His2 zinc finger/TATA box complexes., Wolfe SA, Grant RA, Elrod-Erickson M, Pabo CO, Structure. 2001 Aug;9(8):717-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11587646 11587646] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: zinc finger-dna complex]] | [[Category: zinc finger-dna complex]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:17:14 2008'' |
Revision as of 09:17, 20 March 2008
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STRUCTURE OF A CYS2HIS2 ZINC FINGER/TATA BOX COMPLEX (CLONE #2)
Overview
BACKGROUND: Several methods have been developed for creating Cys2His2 zinc finger proteins that recognize novel DNA sequences, and these proteins may have important applications in biological research and gene therapy. In spite of this progress with design/selection methodology, fundamental questions remain about the principles that govern DNA recognition. One hypothesis suggests that recognition can be described by a simple set of rules--essentially a "recognition code"--but careful assessment of this proposal has been difficult because there have been few structural studies of selected zinc finger proteins. RESULTS: We report the high-resolution cocrystal structures of two zinc finger proteins that had been selected (as variants of Zif268) to recognize a eukaryotic TATA box sequence. The overall docking arrangement of the fingers within the major groove of the DNA is similar to that observed in the Zif268 complex. Nevertheless, comparison of Zif268 and the selected variants reveal significant differences in the pattern of side chain-base interactions. The new structures also reveal side chain-side chain interactions (both within and between fingers) that are important in stabilizing the protein-DNA interface and appear to play substantial roles in recognition. CONCLUSIONS: These new structures highlight the surprising complexity of zinc finger-DNA interactions. The diversity of interactions observed at the protein-DNA interface, which is especially striking for proteins that were all derived from Zif268, challenges fundamental concepts about zinc finger-DNA recognition and underscores the difficulty in developing any meaningful recognition code.
About this Structure
1G2D is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Beyond the "recognition code": structures of two Cys2His2 zinc finger/TATA box complexes., Wolfe SA, Grant RA, Elrod-Erickson M, Pabo CO, Structure. 2001 Aug;9(8):717-23. PMID:11587646
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