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1g7r
From Proteopedia
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| - | [[Image:1g7r.jpg|left|200px]] | + | [[Image:1g7r.jpg|left|200px]] |
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| - | '''X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B''' | + | {{Structure |
| + | |PDB= 1g7r |SIZE=350|CAPTION= <scene name='initialview01'>1g7r</scene>, resolution 2.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1G7R is a [ | + | 1G7R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7R OCA]. |
==Reference== | ==Reference== | ||
| - | X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:[http:// | + | X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11114334 11114334] |
[[Category: Methanothermobacter thermautotrophicus]] | [[Category: Methanothermobacter thermautotrophicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: translational gtpase]] | [[Category: translational gtpase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:19:25 2008'' |
Revision as of 09:19, 20 March 2008
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| , resolution 2.2Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-RAY STRUCTURE OF TRANSLATION INITIATION FACTOR IF2/EIF5B
Overview
X-ray structures of the universal translation initiation factor IF2/eIF5B have been determined in three states: free enzyme, inactive IF2/eIF5B.GDP, and active IF2/eIF5B.GTP. The "chalice-shaped" enzyme is a GTPase that facilitates ribosomal subunit joining and Met-tRNA(i) binding to ribosomes in all three kingdoms of life. The conserved core of IF2/eIF5B consists of an N-terminal G domain (I) plus an EF-Tu-type beta barrel (II), followed by a novel alpha/beta/alpha-sandwich (III) connected via an alpha helix to a second EF-Tu-type beta barrel (IV). Structural comparisons reveal a molecular lever, which amplifies a modest conformational change in the Switch 2 region of the G domain induced by Mg(2+)/GTP binding over a distance of 90 A from the G domain active center to domain IV. Mechanisms of GTPase function and ribosome binding are discussed.
About this Structure
1G7R is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.
Reference
X-Ray structures of the universal translation initiation factor IF2/eIF5B: conformational changes on GDP and GTP binding., Roll-Mecak A, Cao C, Dever TE, Burley SK, Cell. 2000 Nov 22;103(5):781-92. PMID:11114334
Page seeded by OCA on Thu Mar 20 11:19:25 2008
