2wrn

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[[Image:2wrn.png|left|200px]]
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==THE CRYSTAL STRUCTURE OF THE 70S RIBOSOME BOUND TO EF-TU AND TRNA (PART 1 OF 4).==
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<StructureSection load='2wrn' size='340' side='right' caption='[[2wrn]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wrn]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The January 2010 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''70S Ribosomes'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2010_1 10.2210/rcsb_pdb/mom_2010_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WRN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WRN FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=KIR:KIRROMYCIN'>KIR</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=7MG:7N-METHYL-8-HYDROGUANOSINE-5-MONOPHOSPHATE'>7MG</scene>, <scene name='pdbligand=H2U:5,6-DIHYDROURIDINE-5-MONOPHOSPHATE'>H2U</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=YG:WYBUTOSINE'>YG</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wro|2wro]], [[2wrq|2wrq]], [[2wrr|2wrr]], [[1ibk|1ibk]], [[1ibl|1ibl]], [[2uuc|2uuc]], [[2wdk|2wdk]], [[2j02|2j02]], [[1j5e|1j5e]], [[2j00|2j00]], [[1hnx|1hnx]], [[2b9o|2b9o]], [[2wh3|2wh3]], [[2uub|2uub]], [[1vov|1vov]], [[1emi|1emi]], [[1pnx|1pnx]], [[2v46|2v46]], [[2vqf|2vqf]], [[2wh1|2wh1]], [[1n32|1n32]], [[2f4v|2f4v]], [[2jl5|2jl5]], [[1twt|1twt]], [[1qzc|1qzc]], [[2uxd|2uxd]], [[1fka|1fka]], [[1fjg|1fjg]], [[1n36|1n36]], [[2uxc|2uxc]], [[1hnw|1hnw]], [[1l1u|1l1u]], [[1hr0|1hr0]], [[2v48|2v48]], [[2b64|2b64]], [[1xmq|1xmq]], [[2b9m|2b9m]], [[1pn8|1pn8]], [[2wdg|2wdg]], [[1xnr|1xnr]], [[1hnz|1hnz]], [[1dv4|1dv4]], [[1rss|1rss]], [[2wdh|2wdh]], [[1jgq|1jgq]], [[1i94|1i94]], [[1i96|1i96]], [[2uu9|2uu9]], [[1pn7|1pn7]], [[1eg0|1eg0]], [[1ibm|1ibm]], [[2uxb|2uxb]], [[2vqe|2vqe]], [[1g1x|1g1x]], [[1ha3|1ha3]], [[2uua|2uua]], [[1i95|1i95]], [[1xnq|1xnq]], [[1qd7|1qd7]], [[1gix|1gix]], [[1jgo|1jgo]], [[1jgp|1jgp]], [[2c78|2c78]], [[1n34|1n34]], [[1n33|1n33]], [[1pns|1pns]], [[2jl7|2jl7]], [[1yl4|1yl4]], [[2wdm|2wdm]], [[1xmo|1xmo]], [[1i97|1i97]], [[2wrj|2wrj]], [[2wri|2wri]], [[2wrk|2wrk]], [[2wrl|2wrl]]</td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-synthesizing_GTPase Protein-synthesizing GTPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.5.3 3.6.5.3] </span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wrn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wrn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wrn RCSB], [http://www.ebi.ac.uk/pdbsum/2wrn PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wr/2wrn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The ribosome selects a correct transfer RNA (tRNA) for each amino acid added to the polypeptide chain, as directed by messenger RNA (mRNA). Aminoacyl-tRNA is delivered to the ribosome by elongation factor Tu (EF-Tu), which hydrolyzes guanosine triphosphate (GTP) and releases tRNA in response to codon recognition. The signaling pathway that leads to GTP hydrolysis upon codon recognition is critical to accurate decoding. Here, we present the crystal structure of the ribosome complexed with EF-Tu and aminoacyl-tRNA, refined to 3.6 angstrom resolution. The structure reveals details of the tRNA distortion that allows aminoacyl-tRNA to interact simultaneously with the decoding center of the 30S subunit and EF-Tu at the factor-binding site. A series of conformational changes in EF-Tu and aminoacyl-tRNA suggest a communication pathway between the decoding center and the guanosine triphosphatase center of EF-Tu.
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{{STRUCTURE_2wrn| PDB=2wrn | SCENE= }}
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The Crystal Structure of the Ribosome Bound to EF-Tu and Aminoacyl-tRNA.,Schmeing TM, Voorhees RM, Kelley AC, Gao YG, Murphy FV 4th, Weir JR, Ramakrishnan V Science. 2009 Oct 15. PMID:19833920<ref>PMID:19833920</ref>
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===THE CRYSTAL STRUCTURE OF THE 70S RIBOSOME BOUND TO EF-TU AND TRNA (PART 1 OF 4).===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19833920}}
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==About this Structure==
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[[2wrn]] is a 26 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The January 2010 RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''70S Ribosomes'' by David Goodsell is [http://dx.doi.org/10.2210/rcsb_pdb/mom_2010_1 10.2210/rcsb_pdb/mom_2010_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WRN OCA].
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==See Also==
==See Also==
*[[Elongation factor|Elongation factor]]
*[[Elongation factor|Elongation factor]]
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*[[Ribosomal protein S10|Ribosomal protein S10]]
 
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*[[Ribosomal protein S11|Ribosomal protein S11]]
 
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*[[Ribosomal protein S12|Ribosomal protein S12]]
 
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*[[Ribosomal protein S13|Ribosomal protein S13]]
 
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*[[Ribosomal protein S14|Ribosomal protein S14]]
 
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*[[Ribosomal protein S15|Ribosomal protein S15]]
 
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*[[Ribosomal protein S16|Ribosomal protein S16]]
 
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*[[Ribosomal protein S17|Ribosomal protein S17]]
 
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*[[Ribosomal protein S18|Ribosomal protein S18]]
 
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*[[Ribosomal protein S19|Ribosomal protein S19]]
 
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*[[Ribosomal protein S2|Ribosomal protein S2]]
 
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*[[Ribosomal protein S20|Ribosomal protein S20]]
 
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*[[Ribosomal protein S3|Ribosomal protein S3]]
 
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*[[Ribosomal protein S4|Ribosomal protein S4]]
 
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*[[Ribosomal protein S5|Ribosomal protein S5]]
 
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*[[Ribosomal protein S6|Ribosomal protein S6]]
 
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*[[Ribosomal protein S7|Ribosomal protein S7]]
 
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*[[Ribosomal protein S8|Ribosomal protein S8]]
 
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*[[Ribosomal protein S9|Ribosomal protein S9]]
 
*[[Ribosomal protein THX|Ribosomal protein THX]]
*[[Ribosomal protein THX|Ribosomal protein THX]]
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*[[Ribosome|Ribosome]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019833920</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: 70S Ribosomes]]
[[Category: 70S Ribosomes]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]

Revision as of 07:19, 29 September 2014

THE CRYSTAL STRUCTURE OF THE 70S RIBOSOME BOUND TO EF-TU AND TRNA (PART 1 OF 4).

2wrn, resolution 3.60Å

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