2wdh

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[[Image:2wdh.png|left|200px]]
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==STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A-SITE TRNA, DEACYLATED P-SITE TRNA, AND E-SITE TRNA. THIS FILE CONTAINS THE 30S SUBUNIT A-,P-, AND E-SITE TRNAS AND PAROMOMYCIN FOR MOLECULE II.==
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<StructureSection load='2wdh' size='340' side='right' caption='[[2wdh]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2wdh]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2WDH FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5MU:5-METHYLURIDINE+5-MONOPHOSPHATE'>5MU</scene>, <scene name='pdbligand=8AN:3-AMINO-3-DEOXYADENOSINE+5-(DIHYDROGEN+PHOSPHATE)'>8AN</scene>, <scene name='pdbligand=PHA:PHENYLALANINAL'>PHA</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2wdg|2wdg]], [[2wdi|2wdi]], [[2wdj|2wdj]], [[1hr0|1hr0]], [[2v48|2v48]], [[1ibk|1ibk]], [[1xmq|1xmq]], [[2b64|2b64]], [[1ibl|1ibl]], [[2uuc|2uuc]], [[2b9m|2b9m]], [[1pn8|1pn8]], [[1xnr|1xnr]], [[1dv4|1dv4]], [[1hnz|1hnz]], [[1rss|1rss]], [[2j02|2j02]], [[1j5e|1j5e]], [[1jgq|1jgq]], [[2j00|2j00]], [[1hnx|1hnx]], [[2b9o|2b9o]], [[1i94|1i94]], [[1i96|1i96]], [[2uu9|2uu9]], [[1pn7|1pn7]], [[2uub|2uub]], [[1vov|1vov]], [[1eg0|1eg0]], [[1ibm|1ibm]], [[1emi|1emi]], [[2uxb|2uxb]], [[2vqe|2vqe]], [[1g1x|1g1x]], [[1pnx|1pnx]], [[2v46|2v46]], [[2uua|2uua]], [[1i95|1i95]], [[1xnq|1xnq]], [[1qd7|1qd7]], [[1gix|1gix]], [[2vqf|2vqf]], [[1jgo|1jgo]], [[1jgp|1jgp]], [[1n32|1n32]], [[2f4v|2f4v]], [[2jl5|2jl5]], [[1n34|1n34]], [[1n33|1n33]], [[1twt|1twt]], [[2jl7|2jl7]], [[1pns|1pns]], [[1qzc|1qzc]], [[2uxd|2uxd]], [[1fka|1fka]], [[1yl4|1yl4]], [[1n36|1n36]], [[1fjg|1fjg]], [[1xmo|1xmo]], [[2uxc|2uxc]], [[1hnw|1hnw]], [[1i97|1i97]], [[1l1u|1l1u]], [[2wdk|2wdk]], [[2wdl|2wdl]], [[2wdn|2wdn]], [[2wdm|2wdm]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wdh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wdh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wdh RCSB], [http://www.ebi.ac.uk/pdbsum/2wdh PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/2wdh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein synthesis is catalyzed in the peptidyl transferase center (PTC), located in the large (50S) subunit of the ribosome. No high-resolution structure of the intact ribosome has contained a complete active site including both A- and P-site tRNAs. In addition, although past structures of the 50S subunit have found no ordered proteins at the PTC, biochemical evidence suggests that specific proteins are capable of interacting with the 3' ends of tRNA ligands. Here we present structures, at 3.6-A and 3.5-A resolution respectively, of the 70S ribosome in complex with A- and P-site tRNAs that mimic pre- and post-peptidyl-transfer states. These structures demonstrate that the PTC is very similar between the 50S subunit and the intact ribosome. They also reveal interactions between the ribosomal proteins L16 and L27 and the tRNA substrates, helping to elucidate the role of these proteins in peptidyl transfer.
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{{STRUCTURE_2wdh| PDB=2wdh | SCENE= }}
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Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome.,Voorhees RM, Weixlbaumer A, Loakes D, Kelley AC, Ramakrishnan V Nat Struct Mol Biol. 2009 May;16(5):528-33. Epub 2009 Apr 12. PMID:19363482<ref>PMID:19363482</ref>
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===STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A-SITE TRNA, DEACYLATED P-SITE TRNA, AND E-SITE TRNA. THIS FILE CONTAINS THE 30S SUBUNIT A-,P-, AND E-SITE TRNAS AND PAROMOMYCIN FOR MOLECULE II.===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19363482}}
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==About this Structure==
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[[2wdh]] is a 25 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WDH OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein S10|Ribosomal protein S10]]
 
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*[[Ribosomal protein S11|Ribosomal protein S11]]
 
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*[[Ribosomal protein S12|Ribosomal protein S12]]
 
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*[[Ribosomal protein S13|Ribosomal protein S13]]
 
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*[[Ribosomal protein S14|Ribosomal protein S14]]
 
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*[[Ribosomal protein S15|Ribosomal protein S15]]
 
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*[[Ribosomal protein S16|Ribosomal protein S16]]
 
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*[[Ribosomal protein S17|Ribosomal protein S17]]
 
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*[[Ribosomal protein S18|Ribosomal protein S18]]
 
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*[[Ribosomal protein S19|Ribosomal protein S19]]
 
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*[[Ribosomal protein S2|Ribosomal protein S2]]
 
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*[[Ribosomal protein S20|Ribosomal protein S20]]
 
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*[[Ribosomal protein S3|Ribosomal protein S3]]
 
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*[[Ribosomal protein S4|Ribosomal protein S4]]
 
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*[[Ribosomal protein S5|Ribosomal protein S5]]
 
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*[[Ribosomal protein S6|Ribosomal protein S6]]
 
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*[[Ribosomal protein S7|Ribosomal protein S7]]
 
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*[[Ribosomal protein S8|Ribosomal protein S8]]
 
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*[[Ribosomal protein S9|Ribosomal protein S9]]
 
*[[Ribosomal protein THX|Ribosomal protein THX]]
*[[Ribosomal protein THX|Ribosomal protein THX]]
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*[[Ribosome|Ribosome]]
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*[[Ribosome 3D structures|Ribosome 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019363482</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]

Revision as of 09:31, 29 September 2014

STRUCTURE OF THE THERMUS THERMOPHILUS 70S RIBOSOME IN COMPLEX WITH MRNA, PAROMOMYCIN, ACYLATED A-SITE TRNA, DEACYLATED P-SITE TRNA, AND E-SITE TRNA. THIS FILE CONTAINS THE 30S SUBUNIT A-,P-, AND E-SITE TRNAS AND PAROMOMYCIN FOR MOLECULE II.

2wdh, resolution 3.30Å

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