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1gv7
From Proteopedia
Revision as of 09:28, 20 March 2008
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| , resolution 2.10Å | |||||||
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| Sites: | |||||||
| Ligands: | |||||||
| Activity: | Pancreatic ribonuclease, with EC number 3.1.27.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ARH-I, AN ANGIOGENIN/RNASE A CHIMERA
Overview
Angiogenin and ribonuclease A share 33% sequence identity but have distinct functions. Angiogenin is a potent inducer of angiogenesis that is only weakly ribonucleolytic, whereas ribonuclease A is a robust ribonuclease that is not angiogenic. A chimera ("ARH-I"), in which angiogenin residues 58-70 are replaced with residues 59-73 of ribonuclease A, has intermediate ribonucleolytic potency and no angiogenic activity. Here we report a crystal structure of ARH-I that reveals the molecular basis for these characteristics. The ribonuclease A-derived (guest) segment adopts a structure largely similar to that in ribonuclease A, and successfully converts this region from a cell-binding site to a purine-binding site. At the same time, its presence causes complex changes in the angiogenin-derived (host) portion that account for much of the increased ribonuclease activity of ARH-I. Guest-host interactions of this type probably occur more generally in protein chimeras, emphasizing the importance of direct structural information for understanding the functional behavior of such molecules.
About this Structure
1GV7 is a Single protein structure of sequence from Homo sapiens, bos taurus. Full crystallographic information is available from OCA.
Reference
Guest-host crosstalk in an angiogenin-RNase A chimeric protein., Holloway DE, Shapiro R, Hares MC, Leonidas DD, Acharya KR, Biochemistry. 2002 Aug 20;41(33):10482-9. PMID:12173935
Page seeded by OCA on Thu Mar 20 11:28:21 2008
