1h5q
From Proteopedia
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| - | [[Image:1h5q.gif|left|200px]] | + | [[Image:1h5q.gif|left|200px]] |
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| - | '''MANNITOL DEHYDROGENASE FROM AGARICUS BISPORUS''' | + | {{Structure |
| + | |PDB= 1h5q |SIZE=350|CAPTION= <scene name='initialview01'>1h5q</scene>, resolution 1.50Å | ||
| + | |SITE= <scene name='pdbsite=AC1:Ni+Binding+Site+For+Chain+J'>AC1</scene> | ||
| + | |LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> and <scene name='pdbligand=NAP:NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE'>NAP</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Mannitol_2-dehydrogenase_(NADP(+)) Mannitol 2-dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.138 1.1.1.138] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''MANNITOL DEHYDROGENASE FROM AGARICUS BISPORUS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H5Q is a [ | + | 1H5Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Agaricus_bisporus Agaricus bisporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H5Q OCA]. |
==Reference== | ==Reference== | ||
| - | The crystallographic structure of the mannitol 2-dehydrogenase NADP+ binary complex from Agaricus bisporus., Horer S, Stoop J, Mooibroek H, Baumann U, Sassoon J, J Biol Chem. 2001 Jul 20;276(29):27555-61. Epub 2001 May 2. PMID:[http:// | + | The crystallographic structure of the mannitol 2-dehydrogenase NADP+ binary complex from Agaricus bisporus., Horer S, Stoop J, Mooibroek H, Baumann U, Sassoon J, J Biol Chem. 2001 Jul 20;276(29):27555-61. Epub 2001 May 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11335726 11335726] |
[[Category: Agaricus bisporus]] | [[Category: Agaricus bisporus]] | ||
[[Category: Mannitol 2-dehydrogenase (NADP(+))]] | [[Category: Mannitol 2-dehydrogenase (NADP(+))]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:32:37 2008'' |
Revision as of 09:32, 20 March 2008
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| , resolution 1.50Å | |||||||
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| Sites: | |||||||
| Ligands: | and | ||||||
| Activity: | Mannitol 2-dehydrogenase (NADP(+)), with EC number 1.1.1.138 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
MANNITOL DEHYDROGENASE FROM AGARICUS BISPORUS
Overview
Mannitol, an acyclic six-carbon polyol, is one of the most abundant sugar alcohols occurring in nature. In the button mushroom, Agaricus bisporus, it is synthesized from fructose by the enzyme mannitol 2-dehydrogenase (MtDH; EC ) using NADPH as a cofactor. Mannitol serves as the main storage carbon (up to 50% of the fruit body dry weight) and plays a critical role in growth, fruit body development, osmoregulation, and salt tolerance. Furthermore, mannitol dehydrogenases are being evaluated for commercial mannitol production as alternatives to the less efficient chemical reduction of fructose. Given the importance of mannitol metabolism and mannitol dehydrogenases, MtDH was cloned into the pET28 expression system and overexpressed in Escherichia coli. Kinetic and physicochemical properties of the recombinant enzyme are indistinguishable from the natural enzyme. The crystal structure of its binary complex with NADP was solved at 1.5-A resolution and refined to an R value of 19.3%. It shows MtDH to be a tetramer and a member of the short chain dehydrogenase/reductase family of enzymes. The catalytic residues forming the so-called catalytic triad can be assigned to Ser(149), Tyr(169), and Lys(173).
About this Structure
1H5Q is a Single protein structure of sequence from Agaricus bisporus. Full crystallographic information is available from OCA.
Reference
The crystallographic structure of the mannitol 2-dehydrogenase NADP+ binary complex from Agaricus bisporus., Horer S, Stoop J, Mooibroek H, Baumann U, Sassoon J, J Biol Chem. 2001 Jul 20;276(29):27555-61. Epub 2001 May 2. PMID:11335726
Page seeded by OCA on Thu Mar 20 11:32:37 2008
