1hdr
From Proteopedia
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| - | [[Image:1hdr.gif|left|200px]] | + | [[Image:1hdr.gif|left|200px]] |
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| - | '''THE CRYSTALLOGRAPHIC STRUCTURE OF A HUMAN DIHYDROPTERIDINE REDUCTASE NADH BINARY COMPLEX EXPRESSED IN ESCHERICHIA COLI BY A CDNA CONSTRUCTED FROM ITS RAT HOMOLOGUE''' | + | {{Structure |
| + | |PDB= 1hdr |SIZE=350|CAPTION= <scene name='initialview01'>1hdr</scene>, resolution 2.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/6,7-dihydropteridine_reductase 6,7-dihydropteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.34 1.5.1.34] | ||
| + | |GENE= CDNA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | }} | ||
| + | |||
| + | '''THE CRYSTALLOGRAPHIC STRUCTURE OF A HUMAN DIHYDROPTERIDINE REDUCTASE NADH BINARY COMPLEX EXPRESSED IN ESCHERICHIA COLI BY A CDNA CONSTRUCTED FROM ITS RAT HOMOLOGUE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HDR is a [ | + | 1HDR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HDR OCA]. |
==Reference== | ==Reference== | ||
| - | The crystallographic structure of a human dihydropteridine reductase NADH binary complex expressed in Escherichia coli by a cDNA constructed from its rat homologue., Su Y, Varughese KI, Xuong NH, Bray TL, Roche DJ, Whiteley JM, J Biol Chem. 1993 Dec 25;268(36):26836-41. PMID:[http:// | + | The crystallographic structure of a human dihydropteridine reductase NADH binary complex expressed in Escherichia coli by a cDNA constructed from its rat homologue., Su Y, Varughese KI, Xuong NH, Bray TL, Roche DJ, Whiteley JM, J Biol Chem. 1993 Dec 25;268(36):26836-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8262916 8262916] |
[[Category: 6,7-dihydropteridine reductase]] | [[Category: 6,7-dihydropteridine reductase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: oxidoreductase(acting on nadh)]] | [[Category: oxidoreductase(acting on nadh)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:35:51 2008'' |
Revision as of 09:35, 20 March 2008
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| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | CDNA (Homo sapiens) | ||||||
| Activity: | 6,7-dihydropteridine reductase, with EC number 1.5.1.34 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE CRYSTALLOGRAPHIC STRUCTURE OF A HUMAN DIHYDROPTERIDINE REDUCTASE NADH BINARY COMPLEX EXPRESSED IN ESCHERICHIA COLI BY A CDNA CONSTRUCTED FROM ITS RAT HOMOLOGUE
Contents |
Overview
A human dihydropteridine reductase (EC 1.6.99.10) has been created from a rat cDNA clone by a single five-oligonucleotide mutagenesis reaction and expressed in good yield in Escherichia coli. The enzyme has been purified to homogeneity, and kinetic identity to the naturally occurring enzyme has been proven. Crystallization has also been achieved, and the crystal structure was solved using 2.5 A data that was refined to an R value of 16.9%. The structure described in this report represents the first complete structural characterization of this important human enzyme.
Disease
Known diseases associated with this structure: Phenylketonuria due to dihydropteridine reductase deficiency OMIM:[261630]
About this Structure
1HDR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystallographic structure of a human dihydropteridine reductase NADH binary complex expressed in Escherichia coli by a cDNA constructed from its rat homologue., Su Y, Varughese KI, Xuong NH, Bray TL, Roche DJ, Whiteley JM, J Biol Chem. 1993 Dec 25;268(36):26836-41. PMID:8262916
Page seeded by OCA on Thu Mar 20 11:35:51 2008
