1hqc
From Proteopedia
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| - | [[Image:1hqc.gif|left|200px]] | + | [[Image:1hqc.gif|left|200px]] |
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| - | '''STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8''' | + | {{Structure |
| + | |PDB= 1hqc |SIZE=350|CAPTION= <scene name='initialview01'>1hqc</scene>, resolution 3.2Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADE:ADENINE'>ADE</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] | ||
| + | |GENE= RUVB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=274 Thermus thermophilus]) | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HQC is a [ | + | 1HQC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQC OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8., Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7. Epub 2001 Feb 6. PMID:[http:// | + | Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8., Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7. Epub 2001 Feb 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11171970 11171970] |
[[Category: Adenosinetriphosphatase]] | [[Category: Adenosinetriphosphatase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: ruvb]] | [[Category: ruvb]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:40:17 2008'' |
Revision as of 09:40, 20 March 2008
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| , resolution 3.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | RUVB (Thermus thermophilus) | ||||||
| Activity: | Adenosinetriphosphatase, with EC number 3.6.1.3 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8
Overview
We report here the crystal structure of the RuvB motor protein from Thermus thermophilus HB8, which drives branch migration of the Holliday junction during homologous recombination. RuvB has a crescent-like architecture consisting of three consecutive domains, the first two of which are involved in ATP binding and hydrolysis. DNA is likely to interact with a large basic cleft, which encompasses the ATP-binding pocket and domain boundaries, whereas the junction-recognition protein RuvA may bind a flexible beta-hairpin protruding from the N-terminal domain. The structures of two subunits, related by a noncrystallographic pseudo-2-fold axis, imply that conformational changes of motor protein coupled with ATP hydrolysis may reflect motility essential for its translocation around double-stranded DNA.
About this Structure
1HQC is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8., Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K, Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1442-7. Epub 2001 Feb 6. PMID:11171970
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