1ht9

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[[Image:1ht9.gif|left|200px]]<br /><applet load="1ht9" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ht9.gif|left|200px]]
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caption="1ht9, resolution 1.76&Aring;" />
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'''DOMAIN SWAPPING EF-HANDS'''<br />
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{{Structure
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|PDB= 1ht9 |SIZE=350|CAPTION= <scene name='initialview01'>1ht9</scene>, resolution 1.76&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''DOMAIN SWAPPING EF-HANDS'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1HT9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT9 OCA].
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1HT9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HT9 OCA].
==Reference==
==Reference==
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An extended hydrophobic core induces EF-hand swapping., Hakansson M, Svensson A, Fast J, Linse S, Protein Sci. 2001 May;10(5):927-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11316872 11316872]
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An extended hydrophobic core induces EF-hand swapping., Hakansson M, Svensson A, Fast J, Linse S, Protein Sci. 2001 May;10(5):927-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11316872 11316872]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: folding]]
[[Category: folding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:04:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:41:17 2008''

Revision as of 09:41, 20 March 2008

Image:1ht9.gif


PDB ID 1ht9

Drag the structure with the mouse to rotate
, resolution 1.76Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



DOMAIN SWAPPING EF-HANDS


Overview

The structure of calbindin D(9k) with two substitutions was determined by X-ray crystallography at 1.8-A resolution. Unlike wild-type calbindin D(9k), which is a monomeric protein with two EF-hands, the structure of the mutated calbindin D(9k) reveals an intertwined dimer. In the dimer, two EF-hands of the monomers have exchanged places, and thus a 3D domain-swapped dimer has been formed. EF-hand I of molecule A is packed toward EF-hand II of molecule B and vice versa. The formation of a hydrophobic cluster, in a region linking the EF-hands, promotes the conversion of monomers to 3D domain-swapped dimers. We propose a mechanism by which domain swapping takes place via the apo form of calbindin D(9k). Once formed, the calbindin D(9k) dimers are remarkably stable, as with even larger misfolded aggregates like amyloids. Thus calbindin D(9k) dimers cannot be converted to monomers by dilution. However, heating can be used for conversion, indicating high energy barriers separating monomers from dimers.

About this Structure

1HT9 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

An extended hydrophobic core induces EF-hand swapping., Hakansson M, Svensson A, Fast J, Linse S, Protein Sci. 2001 May;10(5):927-33. PMID:11316872

Page seeded by OCA on Thu Mar 20 11:41:17 2008

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