1ibi
From Proteopedia
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| - | [[Image:1ibi.jpg|left|200px]] | + | [[Image:1ibi.jpg|left|200px]] |
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| - | '''QUAIL CYSTEINE AND GLYCINE-RICH PROTEIN, NMR, 15 MINIMIZED MODEL STRUCTURES''' | + | {{Structure |
| + | |PDB= 1ibi |SIZE=350|CAPTION= <scene name='initialview01'>1ibi</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CSRP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=93934 Coturnix japonica]) | ||
| + | }} | ||
| + | |||
| + | '''QUAIL CYSTEINE AND GLYCINE-RICH PROTEIN, NMR, 15 MINIMIZED MODEL STRUCTURES''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IBI is a [ | + | 1IBI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Coturnix_japonica Coturnix japonica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IBI OCA]. |
==Reference== | ==Reference== | ||
| - | Application of cross-correlated NMR spin relaxation to the zinc-finger protein CRP2(LIM2): evidence for collective motions in LIM domains., Schuler W, Kloiber K, Matt T, Bister K, Konrat R, Biochemistry. 2001 Aug 14;40(32):9596-604. PMID:[http:// | + | Application of cross-correlated NMR spin relaxation to the zinc-finger protein CRP2(LIM2): evidence for collective motions in LIM domains., Schuler W, Kloiber K, Matt T, Bister K, Konrat R, Biochemistry. 2001 Aug 14;40(32):9596-604. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11583159 11583159] |
[[Category: Coturnix japonica]] | [[Category: Coturnix japonica]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Schuler, W.]] | [[Category: Schuler, W.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| - | [[Category: lim domain containing | + | [[Category: lim domain containing protein]] |
[[Category: metal-binding protein]] | [[Category: metal-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:48:07 2008'' |
Revision as of 09:48, 20 March 2008
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| Ligands: | |||||||
| Gene: | CSRP2 (Coturnix japonica) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
QUAIL CYSTEINE AND GLYCINE-RICH PROTEIN, NMR, 15 MINIMIZED MODEL STRUCTURES
Overview
The solution structure of quail CRP2(LIM2) was significantly improved by using an increased number of NOE constraints obtained from a 13C,15N-labeled protein sample and by applying a recently developed triple-resonance cross-correlated relaxation experiment for the determination of the backbone dihedral angle psi. Additionally, the relative orientation of the 15N(i)-1HN(i) dipole and the 13CO(i) CSA tensor, which is related to both backbone angles phi and psi, was probed by nitrogen-carbonyl multiple-quantum relaxation and used as an additional constraint for the refinement of the local geometry of the metal-coordination sites in CRP2(LIM2). The backbone dynamics of residues located in the folded part of CRP2(LIM2) have been characterized by proton-detected 13C'(i-1)-15N(i) and 15N(i)-1HN(i) multiple-quantum relaxation, respectively. We show that regions having cross-correlated time modulation of backbone isotropic chemical shifts on the millisecond to microsecond time scale correlate with residues that are structurally altered in the mutant protein CRP2(LIM2)R122A (disruption of the CCHC zinc-finger stabilizing side-chain hydrogen bond) and that these residues are part of an extended hydrogen-bonding network connecting the two zinc-binding sites. This indicates the presence of long-range collective motions in the two zinc-binding subdomains. The conformational plasticity of the LIM domain may be of functional relevance for this important protein recognition motif.
About this Structure
1IBI is a Single protein structure of sequence from Coturnix japonica. Full crystallographic information is available from OCA.
Reference
Application of cross-correlated NMR spin relaxation to the zinc-finger protein CRP2(LIM2): evidence for collective motions in LIM domains., Schuler W, Kloiber K, Matt T, Bister K, Konrat R, Biochemistry. 2001 Aug 14;40(32):9596-604. PMID:11583159
Page seeded by OCA on Thu Mar 20 11:48:07 2008
