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2w6r

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[[Image:2w6r.png|left|200px]]
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==CRYSTAL STRUCTURE OF AN ARTIFICIAL (BA)8-BARREL PROTEIN DESIGNED FROM IDENTICAL HALF BARRELS==
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<StructureSection load='2w6r' size='340' side='right' caption='[[2w6r]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2w6r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W6R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W6R FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a0n|2a0n]], [[1thf|1thf]], [[1gpw|1gpw]], [[1vh7|1vh7]]</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w6r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w6r RCSB], [http://www.ebi.ac.uk/pdbsum/2w6r PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w6/2w6r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ample evidence suggests that the ubiquitous (betaalpha)(8)-barrel enzyme fold has evolved by the duplication and fusion of an ancestral (betaalpha)(4)-half-barrel. To reconstruct this process in the laboratory with a model protein, we earlier fused two copies of the C-terminal half-barrel HisF-C of imidazole glycerol phosphate synthase (HisF) and stepwise stabilized the resulting HisF-CC construct. We now further increased its stability and solubility by introducing two additional amino acid exchanges, which allowed us to crystallize the resulting artificial (betaalpha)(8)-barrel protein HisF-C***C. The analysis of its X-ray structure at 2.1 A resolution reveals a striking similarity to wild-type HisF, helps us to understand its improved stability, and provides further insights into the evolution of (betaalpha)(8)-barrel proteins.
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{{STRUCTURE_2w6r| PDB=2w6r | SCENE= }}
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High-Resolution Crystal Structure of an Artificial (betaalpha)(8)-Barrel Protein Designed from Identical Half-Barrels (dagger) (double dagger).,Hocker B, Lochner A, Seitz T, Claren J, Sterner R Biochemistry. 2009 Jan 23. PMID:19166324<ref>PMID:19166324</ref>
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===CRYSTAL STRUCTURE OF AN ARTIFICIAL (BA)8-BARREL PROTEIN DESIGNED FROM IDENTICAL HALF BARRELS===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19166324}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2w6r]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W6R OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019166324</ref><references group="xtra"/>
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[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
[[Category: Claren, J.]]
[[Category: Claren, J.]]

Revision as of 02:00, 1 October 2014

CRYSTAL STRUCTURE OF AN ARTIFICIAL (BA)8-BARREL PROTEIN DESIGNED FROM IDENTICAL HALF BARRELS

2w6r, resolution 2.10Å

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