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2vqr

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[[Image:2vqr.png|left|200px]]
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==CRYSTAL STRUCTURE OF A PHOSPHONATE MONOESTER HYDROLASE FROM RHIZOBIUM LEGUMINOSARUM: A NEW MEMBER OF THE ALKALINE PHOSPHATASE SUPERFAMILY==
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<StructureSection load='2vqr' size='340' side='right' caption='[[2vqr]], [[Resolution|resolution]] 1.42&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2vqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizobium_leguminosarum_bv._viciae Rhizobium leguminosarum bv. viciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VQR FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DDZ:3,3-DIHYDROXY+L-ALANINE'>DDZ</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vqr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vqr RCSB], [http://www.ebi.ac.uk/pdbsum/2vqr PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vq/2vqr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The alkaline phosphatase superfamily comprises a large number of hydrolytic metalloenzymes such as phosphatases and sulfatases. We have characterised a new member of this superfamily, a phosphonate monoester hydrolase/phosphodiesterase from Rhizobium leguminosarum (R/PMH) both structurally and kinetically. The 1.42 A crystal structure shows structural homology to arylsulfatases with conservation of the core alpha/beta-fold, the mononuclear active site and most of the active-site residues. Sulfatases use a unique formylglycine nucleophile, formed by posttranslational modification of a cysteine/serine embedded in a signature sequence (C/S)XPXR. We provide mass spectrometric and mutational evidence that R/PMH is the first non-sulfatase enzyme shown to use a formylglycine as the catalytic nucleophile. R/PMH hydrolyses phosphonate monoesters and phosphate diesters with similar efficiency. Burst kinetics suggest that substrate hydrolysis proceeds via a double-displacement mechanism. Kinetic characterisation of active-site mutations establishes the catalytic contributions of individual residues. A mechanism for substrate hydrolysis is proposed on the basis of the kinetic data and structural comparisons with E. coli alkaline phosphatase and Pseudomonas aeruginosa arylsulfatase. R/PMH represents a further example of conservation of the overall structure and mechanism within the alkaline phosphatase superfamily.
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{{STRUCTURE_2vqr| PDB=2vqr | SCENE= }}
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A new member of the alkaline phosphatase superfamily with a formylglycine nucleophile: structural and kinetic characterisation of a phosphonate monoester hydrolase/phosphodiesterase from Rhizobium leguminosarum.,Jonas S, van Loo B, Hyvonen M, Hollfelder F J Mol Biol. 2008 Dec 5;384(1):120-36. Epub 2008 Sep 4. PMID:18793651<ref>PMID:18793651</ref>
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===CRYSTAL STRUCTURE OF A PHOSPHONATE MONOESTER HYDROLASE FROM RHIZOBIUM LEGUMINOSARUM: A NEW MEMBER OF THE ALKALINE PHOSPHATASE SUPERFAMILY===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_18793651}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2vqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhizobium_leguminosarum_bv._viciae Rhizobium leguminosarum bv. viciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VQR OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:018793651</ref><references group="xtra"/>
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[[Category: Rhizobium leguminosarum bv. viciae]]
[[Category: Rhizobium leguminosarum bv. viciae]]
[[Category: Hollfelder, F.]]
[[Category: Hollfelder, F.]]

Revision as of 02:04, 1 October 2014

CRYSTAL STRUCTURE OF A PHOSPHONATE MONOESTER HYDROLASE FROM RHIZOBIUM LEGUMINOSARUM: A NEW MEMBER OF THE ALKALINE PHOSPHATASE SUPERFAMILY

2vqr, resolution 1.42Å

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