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2zwn

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[[Image:2zwn.png|left|200px]]
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==Crystal structure of the novel two-domain type laccase from a metagenome==
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<StructureSection load='2zwn' size='340' side='right' caption='[[2zwn]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2zwn]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Metagenomes Metagenomes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZWN FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C2O:CU-O-CU+LINKAGE'>C2O</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2zwn RCSB], [http://www.ebi.ac.uk/pdbsum/2zwn PDBsum]</span></td></tr>
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<table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zw/2zwn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A multi-copper protein with two cupredoxin-like domains was identified from our in-house metagenomic database. The recombinant protein, mgLAC, contained four copper ions/subunits, oxidized various phenolic and non-phenolic substrates, and had spectroscopic properties similar to common laccases. X-ray structure analysis revealed a homotrimeric architecture for this enzyme, which resembles nitrite reductase (NIR). However, a difference in copper coordination was found at the domain interface. mgLAC contains a T2/T3 tri-nuclear copper cluster at this site, whereas a mononuclear T2 copper occupies this position in NIR. The trimer is thus an essential part of the architecture of two-domain multi-copper proteins, and mgLAC may be an evolutionary precursor of NIR.
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{{STRUCTURE_2zwn| PDB=2zwn | SCENE= }}
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X-ray structure of a two-domain type laccase: a missing link in the evolution of multi-copper proteins.,Komori H, Miyazaki K, Higuchi Y FEBS Lett. 2009 Apr 2;583(7):1189-95. Epub 2009 Mar 11. PMID:19285076<ref>PMID:19285076</ref>
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===Crystal structure of the novel two-domain type laccase from a metagenome===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_19285076}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2zwn]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Metagenomes Metagenomes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZWN OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:019285076</ref><references group="xtra"/>
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[[Category: Metagenomes]]
[[Category: Metagenomes]]
[[Category: Higuchi, Y.]]
[[Category: Higuchi, Y.]]

Revision as of 05:12, 2 October 2014

Crystal structure of the novel two-domain type laccase from a metagenome

2zwn, resolution 1.70Å

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