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1izc

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[[Image:1izc.jpg|left|200px]]<br /><applet load="1izc" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1izc.jpg|left|200px]]
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caption="1izc, resolution 1.7&Aring;" />
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'''Crystal Structure Analysis of Macrophomate synthase'''<br />
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{{Structure
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|PDB= 1izc |SIZE=350|CAPTION= <scene name='initialview01'>1izc</scene>, resolution 1.7&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=PYR:PYRUVIC ACID'>PYR</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''Crystal Structure Analysis of Macrophomate synthase'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1IZC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Macrophoma_commelinae Macrophoma commelinae] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=PYR:'>PYR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IZC OCA].
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1IZC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Macrophoma_commelinae Macrophoma commelinae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IZC OCA].
==Reference==
==Reference==
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Insight into a natural Diels-Alder reaction from the structure of macrophomate synthase., Ose T, Watanabe K, Mie T, Honma M, Watanabe H, Yao M, Oikawa H, Tanaka I, Nature. 2003 Mar 13;422(6928):185-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12634789 12634789]
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Insight into a natural Diels-Alder reaction from the structure of macrophomate synthase., Ose T, Watanabe K, Mie T, Honma M, Watanabe H, Yao M, Oikawa H, Tanaka I, Nature. 2003 Mar 13;422(6928):185-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12634789 12634789]
[[Category: Macrophoma commelinae]]
[[Category: Macrophoma commelinae]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: tim-barrel]]
[[Category: tim-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:17:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:57:00 2008''

Revision as of 09:57, 20 March 2008

Image:1izc.jpg


PDB ID 1izc

Drag the structure with the mouse to rotate
, resolution 1.7Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



Crystal Structure Analysis of Macrophomate synthase


Overview

The Diels-Alder reaction, which forms a six-membered ring from an alkene (dienophile) and a 1,3-diene, is synthetically very useful for construction of cyclic products with high regio- and stereoselectivity under mild conditions. It has been applied to the synthesis of complex pharmaceutical and biologically active compounds. Although evidence on natural Diels-Alderases has been accumulated in the biosynthesis of secondary metabolites, there has been no report on the structural details of the natural Diels-Alderases. The function and catalytic mechanism of the natural Diels-Alderase are of great interest owing to the diversity of molecular skeletons in natural Diels-Alder adducts. Here we present the 1.70 A resolution crystal structure of the natural Diels-Alderase, fungal macrophomate synthase (MPS), in complex with pyruvate. The active site of the enzyme is large and hydrophobic, contributing amino acid residues that can hydrogen-bond to the substrate 2-pyrone. These data provide information on the catalytic mechanism of MPS, and suggest that the reaction proceeds via a large-scale structural reorganization of the product.

About this Structure

1IZC is a Single protein structure of sequence from Macrophoma commelinae. Full crystallographic information is available from OCA.

Reference

Insight into a natural Diels-Alder reaction from the structure of macrophomate synthase., Ose T, Watanabe K, Mie T, Honma M, Watanabe H, Yao M, Oikawa H, Tanaka I, Nature. 2003 Mar 13;422(6928):185-9. PMID:12634789

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