2jiy

From Proteopedia

Revision as of 15:30, 30 October 2007

PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M149 REPLACED WITH TRP (CHAIN M, AM149W)

Overview

X-ray crystallography has been used to investigate the extent of, structural changes in mutants of the purple bacterial reaction center that, assemble without a particular ubiquinone or bacteriopheophytin cofactor., In the case of the bacteriopheophytin-exclusion mutant, in which Ala M149, was replaced by Trp (AM149W), the quality of protein crystals was improved, over that seen in previous work by minimizing illumination, time, and, temperature during the purification protocol and carrying out crystal, growth at 4 degrees C after overnight incubation at 18 degrees C. The, X-ray crystal structure of the AM149W mutant, determined to a resolution, of 2.2 A, showed very little change in protein structure despite the, absence of the bacteriopheophytin cofactor. Changes in the electron, ... [(full description)]

About this Structure

2JIY is a [Protein complex] structure of sequences from [Rhodobacter sphaeroides] with FE, PO4, CL, LDA, BCL, BPH, U10, CDL, SPN and D12 as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structural Responses to Cavity-Creating Mutations in an Integral Membrane Protein(,)., Fyfe PK, Potter JA, Cheng J, Williams CM, Watson AJ, Jones MR, Biochemistry. 2007 Aug 21;. PMID:17711306

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