This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1jb7
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1jb7.gif|left|200px]] | + | [[Image:1jb7.gif|left|200px]] |
| - | + | ||
| - | '''DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex''' | + | {{Structure |
| + | |PDB= 1jb7 |SIZE=350|CAPTION= <scene name='initialview01'>1jb7</scene>, resolution 1.86Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= MAC-56A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]), MAC-41A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]) | ||
| + | }} | ||
| + | |||
| + | '''DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1JB7 is a [ | + | 1JB7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JB7 OCA]. |
==Reference== | ==Reference== | ||
| - | DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex., Horvath MP, Schultz SC, J Mol Biol. 2001 Jul 6;310(2):367-77. PMID:[http:// | + | DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex., Horvath MP, Schultz SC, J Mol Biol. 2001 Jul 6;310(2):367-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11428895 11428895] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Sterkiella nova]] | [[Category: Sterkiella nova]] | ||
| Line 18: | Line 27: | ||
[[Category: NA]] | [[Category: NA]] | ||
[[Category: dna hydration]] | [[Category: dna hydration]] | ||
| - | [[Category: dna-protein | + | [[Category: dna-protein interaction]] |
[[Category: quadruplex dna]] | [[Category: quadruplex dna]] | ||
[[Category: sodium ion]] | [[Category: sodium ion]] | ||
[[Category: telomere-binding protein]] | [[Category: telomere-binding protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:01:05 2008'' |
Revision as of 10:01, 20 March 2008
| |||||||
| , resolution 1.86Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | MAC-56A (Sterkiella nova), MAC-41A (Sterkiella nova) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA G-Quartets in a 1.86 A Resolution Structure of an Oxytricha nova Telomeric Protein-DNA Complex
Overview
The Oxytricha nova telomere end binding protein (OnTEBP) recognizes, binds and protects the single-stranded 3'-terminal DNA extension found at the ends of macronuclear chromosomes. The structure of this complex shows that the single strand GGGGTTTTGGGG DNA binds in a deep cleft between the two protein subunits of OnTEBP, adopting a non-helical and irregular conformation. In extending the resolution limit of this structure to 1.86 A, we were surprised to find a G-quartet linked dimer of the GGGGTTTTGGGG DNA also packing within the crystal lattice and interacting with the telomere end binding protein. The G-quartet DNA exhibits the same structure and topology as previously observed in solution by NMR with diagonally crossing d(TTTT) loops at either end of the four-stranded helix. Additionally, the crystal structure reveals clearly visible Na(+), and specific patterns of bound water molecules in the four non-equivalent grooves. Although the G-quartet:protein contact surfaces are modest and might simply represent crystal packing interactions, it is interesting to speculate that the two types of telomeric DNA-protein interactions observed here might both be important in telomere biology.
About this Structure
1JB7 is a Protein complex structure of sequences from Sterkiella nova. Full crystallographic information is available from OCA.
Reference
DNA G-quartets in a 1.86 A resolution structure of an Oxytricha nova telomeric protein-DNA complex., Horvath MP, Schultz SC, J Mol Biol. 2001 Jul 6;310(2):367-77. PMID:11428895
Page seeded by OCA on Thu Mar 20 12:01:05 2008
