1jhi
From Proteopedia
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| - | [[Image:1jhi.gif|left|200px]] | + | [[Image:1jhi.gif|left|200px]] |
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| - | '''Solution Structure of a Hedamycin-DNA complex''' | + | {{Structure |
| + | |PDB= 1jhi |SIZE=350|CAPTION= <scene name='initialview01'>1jhi</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEH:HEDAMYCIN'>HEH</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Solution Structure of a Hedamycin-DNA complex''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JHI is a [ | + | 1JHI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JHI OCA]. |
==Reference== | ==Reference== | ||
| - | Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:[http:// | + | Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11820806 11820806] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Burley, G A.]] | [[Category: Burley, G A.]] | ||
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[[Category: hedamycin]] | [[Category: hedamycin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:03:29 2008'' |
Revision as of 10:03, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of a Hedamycin-DNA complex
Overview
Hedamycin, a member of the pluramycin family of drugs, displays a range of biological responses including antitumor and antimicrobial activity. The mechanism of action is via direct interaction with DNA through intercalation between the bases of the oligonucleotide and alkylation of a guanine residue at 5'-PyG-3' sites. There appears to be some minor structural differences between two earlier studies on the interaction of hedamycin with 5'-PyG-3' sites. In this study, a high-resolution NMR analysis of the hedamycin:d(ACCGGT)2 complex was undertaken in order to investigate the effect of replacing the thymine with a guanine at the preferred 5'-CGT-3' site. The resultant structure was compared with earlier work, with particular emphasis placed on the drug conformation. The structure of the hedamycin:d(ACCGGT)2 complex has many features in common with the two previous NMR structures of hedamycin:DNA complexes but differed in the conformation and orientation of the N,N-dimethylvancosamine saccharide of hedamycin in one of these structures. The preferential binding of hedamycin to 5'-CG-3' over 5'-TG-3' binding sites is explained in terms of the orientation and location of the N,N-dimethylvancosamine saccharide in the minor groove.
About this Structure
1JHI is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural investigation of the hedamycin:d(ACCGGT)2 complex by NMR and restrained molecular dynamics., Owen EA, Burley GA, Carver JA, Wickham G, Keniry MA, Biochem Biophys Res Commun. 2002 Feb 8;290(5):1602-8. PMID:11820806
Page seeded by OCA on Thu Mar 20 12:03:29 2008
Categories: Protein complex | Burley, G A. | Carver, J A. | Keniry, M A. | Owen, E A. | Wickham, G. | HEH | Duplex dna | Hedamycin
