Sandbox ceg1p Steven Paris
From Proteopedia
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== Function of Ceg1p and the Cet1-Ceg1 mRNA Capping Complex == | == Function of Ceg1p and the Cet1-Ceg1 mRNA Capping Complex == | ||
| - | Ceg1p is an mRNA guanylyltransferase in ''Saccharomyces cerevisiae'' that forms a heterotetramer with the RNA triphosphatase cet1p which is called the mRNA capping apparatus. This complex is recruited by RNA polymerase II (RNAP-II) and performs the first two steps in the 5’-guanisine capping mechanism of mRNA. Cet1p hydrolyzes the 5’-triphosphate end of the mRNA, | + | Ceg1p is an mRNA guanylyltransferase in ''Saccharomyces cerevisiae'' that forms a heterotetramer with the RNA triphosphatase cet1p which is called the mRNA capping apparatus. This complex is recruited by RNA polymerase II (RNAP-II) and performs the first two steps in the 5’-guanisine capping mechanism of mRNA. Cet1p hydrolyzes the 5’-triphosphate end of the mRNA, removing a phosphate.<ref>PMID:20159466</ref> The Ceg1p guanylyltransferase then adds a guanosine monophosphate to the 5' end of the mRNA, creating a 5'-5' triphosphate linkage. In the final step, an RNA methyltransferase comes and adds a methyl group to the N7 atom of the guanine, completing the 5'-guanine cap.<ref>PMID:24172134</ref> This 5’ capping is necessary for cells to live in yeast among other organisms. The interactions between Cet1p and Ceg1p are extremely important for inducing capping activity. These interactions both stimulate Ceg1p GMP transfer and help localize the complex in the nucleus where RNAP-II can recruit it.<ref>PMID:24205062</ref> |
== Structure of Ceg1p and Interactions with Cet1p in the Capping Apparatus == | == Structure of Ceg1p and Interactions with Cet1p in the Capping Apparatus == | ||
Revision as of 02:43, 14 October 2014
Ceg1p mRNA Guanylyltransferase
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References
- ↑ Gu M, Rajashankar KR, Lima CD. Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus. Structure. 2010 Feb 10;18(2):216-27. PMID:20159466 doi:10.1016/j.str.2009.12.009
- ↑ Lahudkar S, Durairaj G, Uprety B, Bhaumik SR. A novel role for Cet1p mRNA 5'-triphosphatase in promoter proximal accumulation of RNA polymerase II in Saccharomyces cerevisiase. Genetics. 2014 Jan;196(1):161-76. doi: 10.1534/genetics.113.158535. Epub 2013 Oct, 30. PMID:24172134 doi:http://dx.doi.org/10.1534/genetics.113.158535
- ↑ Takizawa N, Fujiwara T, Yamasaki M, Saito A, Fukao A, Nomoto A, Mizumoto K. The essential role for the RNA triphosphatase Cet1p in nuclear import of the mRNA capping enzyme Cet1p-Ceg1p complex of Saccharomyces cerevisiae. PLoS One. 2013 Oct 30;8(10):e78000. doi: 10.1371/journal.pone.0078000., eCollection 2013. PMID:24205062 doi:http://dx.doi.org/10.1371/journal.pone.0078000
